화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.32, No.6, 529-536, November, 2008
Export Citation
신경재생을 위한 BDNF를 함유한 PLGA 지지체의 제조 및 방출
Preparation and BDNF Release Profile of BDNF-loaded PLGA Scaffolds for Tissue Engineered Nerve Regeneration
김초민, 김순희, 오아영, 김근아, 이일우1, 이종문, 강길선†
  • 전북대학교 융합소재연구센터
  • 1가톨릭대학교 신경외과
Chomin Kim, Soon Hee Kim, A Young Oh, Geun Ah Kim, Il Woo Lee1, John M. Rhee, and Gilson Khang†
  • BK-21 Polymer BIN Fusion Research Team, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Korea
  • 1Department of Neurosurgery, College of Medicine, The Catholic University of Korea, 520-2, Daeheung 2 dong, Jung ku, Daejeon 301-723, Korea
E-mail:
초록
뇌 추출 신경성장인자(BDNF)의 서방성 전달체로써 락타이드-글리콜라이드 공중합체(PLGA) 용액에 탈미네랄화된 골분(DBP) 및 히알루론산(HA)를 균일하게 혼합하여 얼음입자추출법으로 다공성 지지체를 제조하였다. ELISA로 BDNF 방출량을 확인하였으며 SEM으로 방출에 따른 지지체의 다공 특성을 관찰하였다. PLGA지지체와 비교시 DBP/HA/PLGA 지지체에서 지속적으로 일정량이 방출됨을 확인하였으며 BDNF의 양이 증가할수록 빠르고 많은 양이 방출되는 패턴을 보였다. 얼음입자추출법으로 제조된 DBP/HA/PLGA 지지체는 BDNF 등의 수용성 사이토카인의 포접이 용이하고, 생분해성 고분자분해 특성에 의해서 방출이 조절되며, 신경손상부분에 이식시 BDNF가 서방화되어 신경재생에 도움을 줄 것으로 기대된다.
We manufactured poly(L-lactide-co-glycolide)(PLGA) scaffolds impregnated demineralized bone particle(DBP) and hyaluronicacid(HA) by ice-particle leaching method and tested their ability of sustained release of brain derived neurotrophic factor(BDNF). BDNF(50 and 200 ng) mixed with PLGA, DBP/PLGA, HA/PLGA and DBP/HA/PLGA scaffold. The release profiles of BDNF from BDNF loaded scaffolds were assayed using ELISA. Morphological changes of scaffolds by BDNF release were also observed by SEM. BDNF stably and sustainedly released from DBP/HA/PLGA than from PLGA and DBP/PLGA scaffolds. DBP/HA/PLGA scaffolds showed the great structural changes, which demonstrated BDNF release amount from DBP/HA/PLGA scaffolds were highest in all groups. We suggest that BDNF loaded DBP/HA/PLGA scaffold would be very useful for nerve regeneration.
Keywords:brain derived neurotrophic factor;poly(L-lactide-co-glycolide);demineralized bone particle;hyaluronic acid;scaffold;spinal cord regeneration
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