화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.31, No.6, 505-511, November, 2007
Export Citation
히알루론산을 함유한 PLGA 지지체의 제조 및 특성결정
Preparation and Characterization of Hyaluronic Acid Loaded PLGA Scaffold by Emulsion Freeze-Drying Method
고연경, 김순희, 정재수, 박정수, 임지예, 김문석1, 이해방1, 강길선†
  • 전북대학교 BK-21 고분자 BIN융합연구팀
  • 1한국화학연구원 나노생체재료연구팀
Youn Kyung Ko, Soon Hee Kim, Jae Soo Jeong, Jung Soo Park, Ji Ye Lim, Moon Suk Kim1, Hae Bang Lee1, and Gilson Khang†
  • BK-21 Polymer BIN Fusion Research Team, Chonbuk National University, 664-14, Dukjin dong 1Ga, Dukjin Ku, Jeonju 561-756, Korea
  • 1Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P. O. Box 107, Yuseong, Daejeon 305-600, Korea
E-mail:
초록
조직의 재생을 위한 지지체재료의 개발은 조직공학 분야의 연구에 있어서 매우 중요한 요인이다. 히알루론산은 조직을 수복하기 위한 체내이식용 구조물로써 널리 사용되고 있는 천연고분자이며, 이를 이용하여 본 연구에서는 히알루론산을 함유한 락타이드-글리콜라이드 공중합체(PLGA) 다공성 지지체를 유화동결 건조법으로 제조하였다. HA-PLGA 지지체는 수은다공도계, 전자현미경 및 물흡수성을 측정하여 특성을 결정하였다. 제조된 HA-PLGA 지지체의 다공도는 약 96.5%, 전체다공면적은 261 m2/g 이였으며, 세포가 성장하기에 적합한 환경인 다공사이의 상호연결이 전자주사현미경을 통해 관찰되었다. 또한 세포의 생존율과 성장률은 MTT(3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide) 방법을 이용하여 분석하였고, 사이토카인 및 수용성 약물의 방출경향을 확인하기 위하여 과립구-대식세포 집락자극인자(GM-CSF)를 지지체에 담지시킨 후 효소결합 면혁 흡착검사(ELISA)를 이용하여 이들의 방출경향을 확인하였다. 천연/합성 하이브리드 담체로서의 HA/PLGA 담체가 PLGA 단독으로 사용하였을 때와 비교하여 볼 때 세포의 증식이 우수하였고, 이는 히알루론산의 우수한 생체적합성 및 수분보유능력에 기인된 것으로 사료된다.
Poly(lactide-co-glycolide)(PLGA) and hyaluronic acid (HA) has been widely used as biocompatible scaffold materials to regenerate tissue. In this present study, we fabricated microporous PLGA and HA loaded PLGA scaffolds by a emusion freeze-drying method. In order to confirm that the release profile of cytokine or water-soluble drugs, we manufactured the granulocyte macrophage colony stimulating factor(GM-CSF) loaded PLGA and HA-PLGA scaffold. All scaffolds were characterized using scanning electron microscope(SEM), mercury porosimeter and wettability measurement. Cell proliferation and viability were assessed by a 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) test. The porosity of HA-PLGA scaffold was greater than 95% with the total pore area of 261 m2/g. The HA-PLGA scaffold exhibited well interconnected pores to allow greater cell adhesion and prolixferation. It was proven by higher cell viability in the HA-PLGA scaffold than PLGA alone. This may be due to the enhanced natural properties and higher water retention capacity of HA.
Keywords:hyaluronic acid;PLGA;scaffold;emulsion freeze-drying method
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