화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.25, No.6, 893-901, November, 2001
Export Citation
조직공학적 신경재생을 위한 NGF를 함유한 PLA 담체의 제조 및 방출
Preparation and Release Profile of NGF-loaded Polylactide Scaffolds for Tissue Engineered Nerve Regeneration
전은경1, 황혜진2, 강길선2, 3, 이일우4, 이종문5, 이해방1, †
  • 1한국화학연구원 생체의료고분자팀, 대전 305-343
  • 2전북대학교 유기신물질공학과, 전주 561-756
  • 3전북대학교 고분자공학과, 전주 561-756
  • 4가톨릭의대 신경외과, 대전 301-723
  • 5전북대학교 고분자공학과, 대전 301-723
Eun Kyung Jeon1, Hae Jin Whang2, Gilson Khang2, 3, Ilwoo Lee4, John M. Rhee5, and Hai Bang Lee1, †
  • 1Biomaterials Laboratory, Korea Research Institute of Chemical Technology, P. O. Box 107, Yusung, Taejon 305-343, Korea
  • 2Department of New Organic Materials, Chonbuk National University, 664-14, Dukjin Dong 1Ga, Dukjin Ku, Chonju 561-756, Korea
  • 3Department of Polymer Science and Technology, Chonbuk National University, 664-14, Dukjin Dong 1Ga, Dukjin Ku, Chonju 561-756,, Korea
  • 4Department of Neurosurgery, College of Medicine, The Catholic University of Korea, Taeheung 2-dong, Taejon 301-723, Korea
  • 5Department of Polymer Science and Technology, Chonbuk National University, 664-14, Dukjin Dong 1Ga, Dukjin Ku, Chonju 561-756, Korea
E-mail:
초록
조직공학적 신경재생 및 파킨슨씨병 등의 신경퇴행성 질환에서의 치료에 이용 목적으로 신경성장인자(nerve growth factor, NGF)를 생분해성 고분자 담체에 NGF를 서방화시키고자 PLA 담체에 함유시켜 유화동결건조법으로 제조하였다. 제조된 NGF의 방출량은 생체외 pH 7.4, 37 ℃의 PBS 조건하에서 4주 동안 방출실험 하였으며, 함유된 NGF의 활성을 확인하기 위하여 PC-12 세포에 직접 배양하여 확인하였다. 제조되어진 PLA 담체는 열린 셀 구조를 가졌으며, 초기 NGF의 함량이 많을수록 방출량도 증가를 보였으며, 제조과정에서의 NGF의 활성을 확인하기 위하여 PC-12 세포를 배양한 결과 신경돌기가 성장하였다. 본 연구는 생분해성 고분자 특성인 확산과 분해에 의해서 생물학적 활성물질인 NGF의 방출을 조절할 수 있으며, 조직공학적으로 서방화되어 3차원적인 신경재생을 가능케 할 것으로 기대된다.
We developed the nerve growth factor (NGF) loaded poly(L-lactide) (PLA) scaffolds by means of emulsion freeze drying method to the possibility for the application of the nerve regeneration of spinal cord disease and the degeneration in Alzheimer's disease. The release amount of NGF from NGF loaded PLA scaffold were analyzed over a 4 week period in vitro at phosphate buffered saline (PBS), pH 7.4, at 37 ℃. It can be observed the open cell pore structure of porous scaffolds and can be easily controlled the pore structure by the controlling of formulation factors resulting in the controlling of the release rate and the release period. The stability of NGF during the preparation of PLA scaffold was evaluated by comparing the released amounts of total NGF, assayed NGF enzyme . linked immunosorbent assay (ELISA). Released NGF has been found to enhance the neurite sprouting and outgrowth from pheochromocytoma (PC-12) cells. These results suggest that the released NGF from NGF loaded PLA scaffold such as conduit type can be very useful for the nerve regeneration in the neural tissue engineering area.
Keywords:tissue engineering;growth factor delivery;NGF;scaffold;nerve regeneration
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