화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korea Polymer Journal, Vol.9, No.5, 267-276, October, 2001
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Preparation and Characterization of Demineralized Bone Particle Impregnated Poly(L-lactide) Scaffolds
Gilson Khang†, Chong Soo Park, John M. Rhee, Sang Jin Lee1, Young Moo Lee1, Myoung Kyu Choi2, Hai Bang Lee2, and Ilwoo Lee3
  • Department of Polymer Science and Technology, Chonbuk National University, 664-14 Dukjin Dong 1 Ga, Dukjin Ku, Chonju 561-756, Korea
  • 1Department of Industrial Chemistry, Hanyang University, 17 Haengdang Dong, Seongdong Ku, Seoul 133-791, Korea
  • 2Biomaterials Laboratory, Korea Research Institutes of Chemical Technology, P.O.Box 107, Yusung, Taejon 305-606, Korea
  • 3Department of Neurosurgery, Catholic University, Medical College, 520-2 Deaheung Dong, Jung Ku, Taejon 301-723, Korea
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In order to endow with new bioactive functionality from demineralized bone particle (DBP) as natural source to poly(L-lactide) (PLA) synthetic biodegradable polymer, porous DBP/PLA as natural/synthetic composite scaffolds were prepared and compared by means of the emulsion freeze drying and solvent casting/salt leaching methods for the possibility of the application of tissue engineered bone and cartilage. For the emulsion freeze drying method, it was observed that the pore size decreased in the order of 79 μm (PLA control) > 47 μm (20% of DBP) > 23 μm (40% of DBP) > 15 μm (80% of DBP). Porosities as well as specific pore areas decreased with increasing the amount of DBP. It can be explained that DBP acts like emulsifier resulting in stabilizing water droplet in emulsion. For the solvent casting/salt leaching method, a uniform distribution of well interconnected pores from the surface to core region were observed the pore size of 80~70 μm independent with DBP amount. Porosities as well as specific pore areas also were almost same. For pore size distribution by the mercury intrusion porosimeter analysis between the two methods, the pore size distribution of the emulsion freeze drying method was broader than that of the solvent casting/salt leaching method due to the mechanism of emulsion formation. Scaffolds of PLA alone, DBP/PLA of 40 and 80%, and DBP powder were implanted on the back of athymic nude mouse to observe the effect of DBP on the induction of cells proliferation by hematoxylin and eosin staining for 8 weeks. It was observed that the effect of DBP/PLA scaffolds on bone induction are stronger than PLA scaffolds, even though the bone induction effect of DBP/PLA scaffold might be lowered than only DBP powder, that is to say, in the order of DBP only > DBP/PLA scaffolds of 40 and 80% DBP > PLA scaffolds only for osteoinduction activity. In conclusion, it seems that DBP plays an important role for bone induction in DBP/PLA scaffolds for the application of tissue engineering area.
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