화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.29, No.3, 282-287, May, 2005
Export Citation
얼음입자추출법을 이용한 알부민 함유 PLGA 담체의 제조 및 방출 거동
Preparation and Release Behavior of Albumin-Loaded PLGA Scaffold by Ice Particle Leaching Method
홍금덕1, 서광수1, 김순희2, 김선경2, 강길선1, 3, †, 신형식4, 김문석3, 이해방3
  • 1전북대학교 고분자ㆍ나노공학과
  • 2전북대학교 유기신물질공학과
  • 3한국화학연구원 나노생체재료고분자팀
  • 4전북대학교 화학공학부
Keum Duck Hong1, Kwang Su Seo1, Soon Hee Kim2, Sun Kyung Kim2, Gilson Khang1, 3, †, Hyung Sik Shin4, Moon Suk Kim3, and Hai Bang Lee3
  • 1Department of PolymerNano Science and Technology, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Korea
  • 2Department of Advanced Organic Materials Engineering, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Korea
  • 3Nanobiomaterials Laboratories, Korea Research Institute of Chemical Technology, P. O. Box 107, Yuseong, Daejon 305-600, Korea
  • 4Department of Chemical Engineering, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Korea
E-mail:
초록
조직공학적 장기재생에 있어서 필수적 요소인 생분해성 담체를 제조하기 위하여 새로운 방법인 얼음입자추출법을 사용하였다. 형광이 결합된 소 혈청 알부민(bovine serum albumin-fluorescein isothiocyanate, FITC-BSA)과 락타이드-글리콜라이드 공중합체(PLGA)를 균일하게 혼합한 후에 제조된 얼음입자를 각각 다른 양을 첨가하여 다공성의 담체를 제조하였다. 모델 약물로 이용한 알부민의 방출 실험은 pH 7.4 인산염완충액을 사용하여 37 ℃, 100 rpm 조건으로 항온조에서 28일 동안 수행하였다. 알부민의 방출은 형광 분광기를 통하여 FITC의 강도에 의해 결정되었으며 알부민의 방출 거동에 따른 담체의 형태학적 변화는 전자주사현미경을 이용하여 관찰하였다. 담체를 알부민이 용해된 용액에 단순히 함침시킨 방법에 비해 알부민을 함유하여 제조한 담체의 경우에 초기 방출량이 적고 일정한 방출거동을 보였다. 또한 알부민의 농도에 따른 실험에서 농도가 증가하여도 초기 방출량은 증가하지 않음을 확인할 수 있었다. 본 실험을 통해 PLGA를 이용하여 얼음입자 추출법으로 제조한 담체는 단백질 약물의 서방호가 훌륭하여 생체조직공학적 담체로서 응용 가능함을 확인하였다. 또한, 물 등에 의한 다공물질 추출 과정이 없기 때문에 사이토카인 등과 같은 수용성 인자들의 포접이 용이하여 조직공학적 바이오장기 재생에 유효할 것으로 사료된다.
A novel ice particle leaching method for fabrication of porous and biodegradable PLGA scaffold has been proposed for the application to tissue engineering. After uniform mixing of poly(L-lactide-co-glycolide) (PLGA) and bovine serum albumin-fluorescein isothiocyanate (FITC-BSA), the FITC-BSA loaded scaffold was fabricated by adding various ratio of ice particle. The release profiles of FITC-BSA were examined using pH 7.4 PBS for 28 days at 37 . The release amount was determined by fluorescence intensity by using the fluorescence spectrophotometer and the morphological change of the scaffolds was observed by scanning electron microscope. The release initial burst of BSA containing scaffolds was lower than that of simple dipping scaffolds resulting in constant release aspect. Although the BSA concentration increased, the initial burst was not increased. As a result of this study, it can be suggested that ice particle leaching method for the tissue engineered scaffold might be very useful and it is possible to impregnate with water soluble factors like cytokine. We suggest that ice particle leaching method may be useful to tissue engineered organ regeneration.
Keywords:ice particle leaching method;PLGA scaffold;FITC-BSA;constant-release;organ regeneration
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