화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.28, No.4, 328-334, July, 2004
Export Citation
알부민을 함유한 메톡시 폴리(에틸렌 글리콜)-폴리(L-락타이드-co-글리콜라이드) 웨이퍼의 제조 및 방출거동
Preparation and Release Behavior of Methoxy poly(ethylene glycol)-poly(L-lactide-co-glycolide) Wafer Containing Albumin
서광수, 김문석1, 김경자2, 조선행1, 이해방1, 강길선†
  • 전북대학교 고분자ㆍ나노공학과
  • 1한국화학연구원 나노생체재료연구팀
  • 2요업(세라믹)기술원 나노연구본부
Kwang Su Seo, Moon Suk Kim1, Kyungja Kim2, Sun Hang Cho1, Hai Bang Lee1, and Gilson Khang†
  • Department of PolymerㆍNano Science and Technology, Chonbuk national University, 664-14, Dukjin Ku, Cheonju 561-756, Korea
  • 1Nanobiomaterials Laboratories, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejon 305-343, Korea
  • 2Biomaterials Laboratories, Korea Institute of Ceramic Engineering & Technolgy, 233-5, Gassan-Dong, Seoul 153-801, Korea
E-mail:
초록
단백질 전달체로 이용하기 위한 폴리(에틸렌 글리콜)-폴리(락타이드-co-글리콜라이드) 공중합체는 카비톨(134 g/mole)과 550, 2000및 5000 g/mole의 각기 다른 분자량을 가진 메톡시 폴리(에틸렌 글리콜)을 개시제로 하여 촉매인 Sn(Oct)2를 사용하여 L-락타이드와 글리콜라이드의 개환중합에 의해 합성되었다. 1H-NMR, GPC, 그리고 XRD를 이용하여 합성된 블록 공중합체의 특성을 결정하였다. 이식형 웨이퍼를 준비하기 위하여 동결분쇄기를 이용하여 형광이 결합된 1%소 혈청 알부민과 고분자를 균일 혼합 후에 3 mm × 1 mm의 크기로 웨이퍼를 제조하였다. 형광 알부민의 방출 거동과 pH변화는 pH 7.4의 인산염완충용액을 이용하여 37 ℃, 100 rpm의 항온조에서 30일 동안 관찰하였다. 알부민의 방출양은 형광분광기를 통하여 FITC의 강도에 의해 결정되었다. 알부민의 방출 거동은 블록 공중합체내에서 MPEG의 분자량이 증가할수록 빠른 초기 방출을 보였고, PLGA의 분자량을 조절함으로서 약물의 방출기간을 결정할 수 있었다. 이러한 결과로 생분해성 PLGA에 친수성인 MPEG의 도입을 통해 약물의 방출거동을 조절할 수 있었다.
A series of methoxy poly(ethylene glycol) (MPEG)-poly(L-lactide-co-glycolide) (PLGA) diblock copolymers were synthesized by ring-opening polymerization of L-lactide and glycolide with carbitol (134 g/mole) or different molecular weights of MPEG (550, 2000, and 5000 g/mole) as an initiator in presence of Sn(Oct)2. The properties of diblock copolymers were characterized by using 1H-NMR, GPC, and XRD. After uniform mixing of block copolymers and 1% albumin bovine-fluorescein isothiocyanate (FITC-BSA) with a freeze miller, the wafers loaded FITC-BSA were fabricated by using a mold with a dimensions of 3 mm × 1mm diameter. The release profiles of FITC-BSA and the pH changes of wafer were examined using pH 7.4 PBS for 30 days at 37 ℃. The release profiles of albumin showed fast initial burst as the molecular weights of MPEG increased. As a result of this study, the release behavior of BSA was controlled with introducing MPEG in the block copolymers.
Keywords:block copolymer;MPEG-PLGA;albumin;protein carrier;wafer
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