화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.11, No.5, 334-340, October, 2003
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Controlled Release of Nerve Growth Factor from Sandwiched Poly(L-lactide-co-glycolide) Films for the Application in Neural Tissue Engineering
Gilson Khang†, Eun Kyung Jeon, John M. Rhee, Ilwoo Lee1, Sang Jin Lee2, and Hai Bang Lee2
  • Department of Polymer·Nano Science and Technology, Chonbuk National University, 664-14, Dukjin Dong 1 Ga, Dukjin Ku, Jeonju 561-756, Korea
  • 1Department of Neurosurgery, Catholic University Medical College, 520-2 Deaheung Dong, Jung Ku, Daejeon 301-723, Korea
  • 2Biomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Korea
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In order to fabricate new sustained delivery device of nerve growth factor (NGF), we developed NGFloaded biodegradable poly(L-lactide-co-glycolide) (PLGA, the mole ratio of lactide to glycolide 75:25, molecular weight: 83,000 and 43,000 g/mole, respectively) film by novel and simple sandwich solvent casting method for the possibility of the application of neural tissue engineering. PLGA was copolymerized by direct condensation reaction and the molecular weight was controlled by reaction time. Released behavior of NGF from NGF-loaded films was characterized by enzyme linked immunosorbent assay (ELISA) and degradation characteristics were observed by scanning electron microscopy (SEM) and gel permeation chromatography (GPC). The bioactivity of released NGF was identified using a rat pheochromocytoma (PC-12) cell based bioassay. The release of NGF from the NGF-loaded PLGA films was prolonged over 35 days with zero-order rate of 0.5~0.8 ng NGF/day without initial burst and could be controlled by the variations of molecular weight and NGF loading amount. After 7 days NGF released in phosphate buffered saline and PC-12 cell cultured on the NGF-loaded PLGA film for 3 days. The released NGF stimulated neurite sprouting in cultured PC-12 cells, that is to say, the remained NGF in the NGF/PLGA film at 37 ℃ for 7 days was still bioactive. This study suggested that NGF-loaded PLGA sandwich film is released the desired period in delivery system and useful neuronal growth culture as nerve contact guidance tube for the application of neural tissue engineering.
Keywords:NGF;PLGA;film;sandwich solvent casting method;NGF release;PC-12 cell
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