화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.25, No.1, 124-128, January, 2008
DOI10.1007/s11814-008-0022-5  Export Citation
Asymmetric reduction of chloroacetophenones to produce chiral alcohols with microorganisms
Zhimin Ou1, 2, †, Jianping Wu1, 3, Lirong Yang1, and Peilin Cen1
  • 1Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou, 310027, China
  • 2Pharmaceuticals College, Zhejiang University of Technology, Hangzhou, 310014, China
  • 3The Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi 214036, China
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Four strains of yeast with reduction activity of chloroacetophenones were screened, in which Saccharomyces cerevisiae B5 showed best reduction activity and stereoselectivity. High optical purity (R)-2'-chloro-1-phenylethanol can be obtained with Saccharomyces cerevisiae B5 as biocatalyst. The influence of several co-substrates on the enantiometric excess (ee%) and yield of (R)-2'-chloro-1-phenylethanol was evaluated. 5% (v/v) ethanol is optimal cosubstrate for (R)-2'-chloro-1-phenylethanol formation. The optimal bioconversion conditions of 2'-chloroacetophenone catalyzed by Saccharomyces cerevisiae B5 are as follows: pH 8.0, 25 oC and 24 h. The yield and the enantiometric excess of (R)-2'-chloro-1-phenylethanol can both reach more than 99% with 10.75 g/l Saccharomyces cerevisiae B5 (the cell dry weight) and 1 g/l 2'-chloroacetophenone used in the biotransformation.
Keywords:Chloroacetophenones;2'-Chloroacetophenone;Saccharomyces cerevisiae B5;(R)-2'-Chloro-1-Phenylethanol
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