Korean Journal of Chemical Engineering, Vol.21, No.1, 201-205, January, 2004
Penicillium sp. ZD-Z1 Chitosanase Immobilized on DEAE Cellulose by Cross-linking Reaction
E-mail:
Chitosanase obtained from Penicillium sp.ZD-Z1 was immobilized on DEAE cellulose with glutaraldehyde by cross-linking reaction. The optimal conditions of immobilization were as follows: 0.1 g DEAE cellulose was treated with 5 ml 5% glutaraldehyde solution; then 2.3 mg chitosanase was immobilized on the carrier. The optimal temperature and pH was 60 ℃ and 4.0, and the Km value was 18.87 g/L. Under optimal conditions, the activity of immobilized enzyme is 1.5 U/g, and the recovery of enzyme activity is 81.3%. After immobilization, the optimal temperature and Km value increased (from 50 ℃ to 60 ℃, from 2.49 g/L to 18.87 g/L), whereas the optimal pH was reduced (from 5.0 to 4.0). The enzyme activity loss was less than 20% after 10 times batch reaction; the immobilized enzyme showed good operation stability.
-
Ilyina AV, Tatarinova NY, Varlamov VP, Process Biochem., 34(9), 875 (1999)
- Allan GR, Hadwiger LA, Exp. Myco., 3, 285 (1979)
- Goldstein L, Levin Y, Katchalski E, Biochemistry, 3, 1913 (1964)
- Hadwiger LA, Beckman JM, Plant Physiol., 66, 205 (1980)
- Hirano S, Itakura C, Seino H, Akiyama Y, Nonaka I, Kanbara N, Kawakami T, J. Agric. Food Chem., 38, 1214 (1990)
- Hirano S, Nagao N, Agric. Biol. Chem., 53, 3065 (1989)
- Kendra DF, Christian D, Hadwiger LA, Physiol. Mol. Plant Patho., 35, 215 (1989)
- Klibanov AM, Anal. Biochem., 93, 1 (1979)
- Kusano S, Shiraishi T, Takahashi S, Fujimoto D, Sakano Y, J. Ferment. Bioeng., 68, 233 (1989)
- Maezaki Y, Tsuji K, Nakagawa Y, Kawai Y, Akimoto M, Tsugita T, Takekawa W, Terada A, Hara H, Mitsoka T, Biosci. Biotechnol. Biochem., 57, 1439 (1993)
- Martinek K, Mozaev VV, Berezin IV, Enzyme Eng., 3, 54 (1980)
- Mehta PK, Miahras S, Ghose TK, Biotechnol. Biochem., 37, 551 (1991)
- Okuda H, Kato H, Tsujita T, Korean J. Chitin Chitosan, 2, 49 (1997)
- Sugano M, Fujikawa T, Hiratsuji Y, Nakashima K, Fukuda N, Hasegawa Y, Am. J. Clin. Nutr., 33, 787 (1980)
- Sugano M, Watanabe S, Kishi A, Izume M, Ohtakara A, Lipids, 23, 187 (1988)
- Sugano M, Yoshida K, Hashimoto M, Enomoto K, Hirano S, "Hypocholesterolemic Activity of Partially Hydrolyzed Chitosans in Rats," In: Brine, C.J., Sanford, P.A. and Zikakis, J.P., editors. Advances in Chitin and Chitosan. Barking, UK: Elsevier Applied Science, 472 (1992)
- Sun SQ, Shao DM, J. Hebei Academy Sci., 2, 35 (1997)
- Suzuki K, Mikami T, Okawa Y, Tokoro A, Suzuki S, Suzuki M, Carbohyd. Res., 151, 403 (1986)
- Suzuki S, Fragrance J., 15, 61 (1996)
- Suzuki S, Watanabe T, Mikami T, Matsumoto T, Suzuki M, "Immuno-enhancing Effects of N-acetylchitohexaose," In: Brine, C.J., Sanford, P.A., Zikakis, J.P., Editors. Advances in Chitin and Chitosan. Barking, UK: Elsevier Applied Science, 96 (1992)
- Tokoro A, Tatewaki N, Suzuki K, Mikami T, Suzuki S, Suzuki M, Chem. Pharm. Bull., 36, 784 (1988)
- Tokoro A, Kobayashi M, Tatekawa N, Suzuki K, Okawa Y, Microbiol. Immunol., 33, 357 (1989)
- Tsukada K, Matsumoto T, Aizawa K, Tokoro A, Naruse R, Suzuki S, Suzuki M, Jpn. J. Cancer. Res., 81, 259 (1990)
- Uchida Y, Izume M, Ohtakara A, "Preparation of Chitosan Oligomers with Purified Chitosanase and its Application," In: SkjakBraek, G., Anthonsen, T. and Sandford, P., Editors. Chitin and Chitosan. Barking, UK: Elsevier Applied Science, 373 (1989)
- Walker SM, Hadwiger L, Ryan CA, Biochem. Biophys. Res. Commun., 110, 194 (1983)
- Yamada A, Shibbuya N, Kodama O, Akatsuka T, Biosci. Biotechnol. Biochem., 57, 405 (1993)