화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.79, 236-244, November, 2019
DOI10.1016/j.jiec.2019.06.044  Export Citation
Influence of fiber architecture and growth factor formulation on osteoblastic differentiation of mesenchymal stem cells in coacervate-coated electrospun fibrous scaffolds
Sungjun Kim1, Jinkyu Lee2, Mintai P. Hwang3, Yadong Wang3, and Kyobum Kim1, 4, †
  • 1Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, Republic of Korea
  • 2Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul, Republic of Korea
  • 3Meinig School of Biomedical Engineering, Cornell University, Ithaca, USA
  • 4Division of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University, Incheon, Republic of Korea
E-mail:
In order to achieve effective bone regeneration, the architectural/structural and biological environment must be considered. In this study, we investigated the influence of structure of extracellular matrixmimicking electrospun poly (L-lactic acid) (PLLA) fibrous scaffolds and incorporated growth factors (bone morphogenetic protein2 (BMP2) and platelet-derived growth factor (PDGF)) (GFs) on in vitro differentiation of human mesenchymal stem cells (hMSCs) cultured onto the scaffolds. Furthermore, Cargo GFs were first encapsulated into coacervate (Coa). Coa-coated nano-sized PLLA (Coa-nPLLA) exhibited relatively higher coating efficacy, than Coa-coated micro-sized PLLA (Coa-mPLLA) due to large mesh hole area of mPLLA. Consequently, a faster GF release pattern was observed in nPLLA groups. As a result of osteogenic differentiation of hMSC which cultured onto GFs loaded Coa-PLLA, the architectural cue of fiber diameters effectively modulated early osteogenic differentiation of hMSCs. Facilitated longterm differentiation of hMSCs could be achieved by synergistic effect of dual BMP2 and PDGF in nanofbrous environments.
Keywords:Poly(L-lactic acid);Fibrous scaffold;Coacervate;Bone morphogenetic protein 2;Platelet-derived growth factor;Osteogenic differentiation
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