Preparation of Polydopamine-Modified 3D Interconnected Macroporous Silica for Laccase Immobilization

Dali Cao; Wenjing Cheng; Kai Tao; Yunxiao Liang

Macromolecular Research, Vol.26, No.7, 616-622, July, 2018

DOI10.1007/s13233-018-6087-z Export Citation

Preparation of Polydopamine-Modified 3D Interconnected Macroporous Silica for Laccase Immobilization

Dali Cao, Wenjing Cheng, Kai Tao, and Yunxiao Liang^†

State Key Lab. Base of Novel Functional Materials and Preparation Science, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, P. R. China

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Millimeter-sized polydopamine (PDA) modified three-dimensional interconnected macroporous silica (PDA/3DIMS) with high porosity, large pore volume and pore diameter was prepared and used as the support to immobilize commercial laccase (Lac) from Denilite II S. The skeleton of 3DIMS is constructed by silica nanofilm, so the PDA/3DIMS is constructed by PDA/SiO2/PDA sandwich nano-film. The immobilization conditions, incubation time, pH and enzyme concentration were optimized, and the properties of the immobilized laccase were investigated. The enzyme activity of Lac-PDA/3DIMS (295.9 U/g) was higher than that of Lac-3DIMS (222.2 U/g). The stability and reusability of the two immobilized laccases were both improved comparing with the free laccase. Lac-PDA/3DIMS exhibited higher retained activity (73.6%) than Lac-3DIMS (31.8%) after reused for ten times. Lac-PDA/3DIMS retained more than 84.0% initial activity after storage for a month, however, the free laccase and Lac-3DIMS were 7.1% and 48.6%, separately. These advantages revealed that PDA thin layer plays an important role to improve the enzymatic activity of immobilized laccase, and the PDA/3DIMS material is a very promising support for enzyme immobilization.

Keywords:laccase;immobilization;three-dimensional interconnected macroporous silica;nano-film

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[4] Rodriguez CS, Herrera JLT, Biotechnol. Adv., 24, 500 (2006)

[5] Fukuda T, Uchida H, Takashima Y, Uwajima T, Kawabata T, Suzuki M, Biochem. Biophys. Res. Commun., 284(3), 704 (2001)

[6] Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R, Enzyme Microb. Technol., 40(6), 1451 (2007)

[7] Qiu H, Xu C, Huang X, Ding Y, Qu B, Gao P, J. Phys. Chem. C, 112, 14781 (2008)

[8] Zhang C, Hu CY, Zhao YL, Moller M, Yan KL, Zhu XM, Langmuir, 29(49), 15457 (2013)

[9] Kaspar O, Tokarova V, Nyanhongo GS, Gubitz G, Stepanek F, Food Bioprod. Process., 91, 525 (2013)

[10] Kawachi Y, Kugimiya S, Nakamura H, Kato K, Appl. Surf. Sci., 314, 64 (2014)

[11] Park JH, Xue H, Jung JS, Ryu K, Korean J. Chem. Eng., 29(10), 1409 (2012)

[12] Sandu T, Sarbu A, Damian CM, Patroi D, Iordache TV, Budinova T, Tsyntsarski B, Yardim MF, Sirkecioglu A, React. Funct. Polym., 96, 5 (2015)

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[14] Pan T, Sun YJ, Wang XL, Shi T, Zhao YL, Chinese Chem. Lett., 25, 983 (2014)

[15] Zheng XB, Wang Q, Jiang YJ, Gao J, Ind. Eng. Chem. Res., 51(30), 10140 (2012)

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[18] Haeshin L, Lee BP, Messersmith PB, Nature, 448, 338 (2007)

[19] Cao Y, Zhang X, Lei T, Li K, Xue Z, Feng L, Wei Y, ACS Appl. Mater. Interfaces,, 5, 4438 (2013)

[20] Li B, Liu T, Hu L, Wang Y, Gao L, ACS Sustain. Chem. Eng., 1, 374 (2013)

[21] Spinelli D, Fatarella E, Di Michele A, Pogni R, Process Biochem., 48(2), 218 (2013)

[22] Kumari A, Kayastha A, J. Mol. Catal. B-Enzym., 69, 8 (2011)

[23] Talbert J, Goddard J, Colloids Surf. B: Biointerfaces, 93, 8 (2012)

[24] Martinez AG, Fibikar S, Santos IP, Marzan LM, Cola LD, Angew. Chem.-Int. Edit., 48, 1266 (2009)

[25] Yu B, Liu J, Liu S, Zhou F, Chem. Commun., 46, 5900 (2010)

[26] Wang F, Guo C, Yang LR, Liu CZ, Bioresour. Technol., 101(23), 8931 (2010)

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[31] Yavuz H, Bayramoglu G, Kacar Y, Denizli A, Arica MY, Biochem. Eng. J., 10, 1 (2002)

[32] Cheng J, Zhuang W, Tang CL, Chen Y, Wu JL, Guo T, Ying HJ, Bioprocess. Biosyst. Eng., 40, 331 (2017)

[33] Ran X, Chi C, Li F, Zhang B, ACS Appl. Mater. Interfa, 5, 12554 (2013)

[34] Dai YR, Niu JF, Liu J, Yin LF, Xu JJ, Bioresour. Technol., 101(23), 8942 (2010)

[35] Sathishkumar P, Chae JC, Unnithan AR, Palvannan T, Kim HY, Lee KJ, Cho M, Kamala-Kannan S, Oh BT, Enzyme Microb. Technol., 51(2), 113 (2012)

[36] Sari M, Akgol S, Karatas M, Denizli A, Ind. Eng. Chem. Res., 45(9), 3036 (2006)

[37] Zhu Y, Kaskel S, Shi J, Wage T, Pee K, Chem. Mater., 19, 6408 (2007)