Korean Journal of Chemical Engineering, Vol.29, No.10, 1409-1412, October, 2012
Immobilization of laccase on carbon nanomaterials
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Laccase from Trametes versicolor was readily immobilized on carbon nanomaterials including multiwalled carbon nanotubes (MWNTs), carboxylated multiwalled carbon nanotubes (MWNT-COOHs), and graphene oxides (GOs), by physical adsorption without using coupling agents. The immobilized amount of laccase strongly depends on the pH of the aqueous buffers of the immobilization mixture. As the pH of the aqueous buffer for immobilization increases, the immobilized amount of laccase decreases. The activity of the immobilized laccase on the three carbon nanomaterials exhibits a bell-shaped dependence on the pH of the immobilization solution with maximum activity at pH 6 or 7. When the immobilization solution becomes acidic or basic, the activity of the immobilized laccase declines significantly. The amount and the activity of immobilized laccase were maximum for graphene oxides as substrate material for immobilization.
- Al-Zuhair S, Biotechnol. Prog., 21(5), 1442 (2005)
- Lee HK, Lee JK, Kim MJ, Lee CJ, Bull. Korean Chem.Soc., 31, 650 (2010)
- Bunte C, Prucker O, Konig T, Ruhe J, Langmuir, 26(8), 6019 (2010)
- Kwan RCH, Chan CY, Renneberg R, Biotechnol. Lett., 24(14), 1203 (2002)
- Shan D, Mousty C, Cosnier S, Anal. Chem., 76, 178 (2004)
- Barton SC, Gallaway J, Atanassov P, Chem. Rev., 104(10), 4867 (2004)
- Endo M, Hayashi T, Kim YA, Pure Appl. Chem., 78, 1703 (2006)
- Luo H, Shi Z, Li N, Gu Z, Zhuang Q, Anal. Chem., 73, 915 (2001)
- Zhang JL, Zhang F, Yang HJ, Huang XL, Liu H, Zhang JY, Guo SW, Langmuir, 26(9), 6083 (2010)
- Jung SK, Chae YR, Yoon JM, Cho BW, Ryu KG, J.Microbiol. Biotechnol., 15, 234 (2005)
- Lee YM, Kwon OY, Yoon YJ, Ryu K, Biotechnol. Lett., 28(1), 39 (2006)
- Hong H, Tayhas G, Palmer R, J. Phys. Chem., 109, 19278 (2005)
- Bourbonnais R, Paice MG, Appl. Microbiol. Biotechnol., 36, 823 (1992)
- Ivnitski D, Atanassov P, Electroanalysis., 19, 2307 (2007)
- Pham VH, Cuong TV, Hur SH, Shin EW, Kim JS, Chung JS, Kim EJ, Carbon., 48, 1945 (2010)
- Zhang HB, Zhang YL, Huang F, Gao PJ, Chen JC, Biotechnol. Lett., 31(6), 837 (2009)
- Madhavi V, Lele SS, AAPG Bull., 4, 1694 (2009)
- Liu Y, Huang L, Dong S, Biosens. Bioelectron., 23, 35 (2007)
- Piontek K, Antorini M, Choinowski T, J. Biol. Chem., 277, 37663 (2002)
- Yaver DS, Xu F, Golightly EJ, Brown KM, Brown SH, Rey MW, Schneider P, Halkier T, Mondorf K, Dalboge H, Appl.Environ. Microbiol., 62, 834 (1996)
- Zhu YW, Murali S, Cai WW, Li XS, Suk JW, Potts JR, Ruoff RS, Adv. Mater., 22(35), 3906 (2010)