화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.41, No.5, 777-783, September, 2017
DOI10.7317/pk.2017.41.5.777  Export Citation
Mineral Oil을 활용한 다공성 키토산 지지체의 물리적 특성 조절에 관한 연구
Study on the Manipulation of Physical Properties of Chitosan Scaffolds Using Mineral Oil
김주승1, 정은진1, 김지혜1, 이우걸1, 2, †
  • 1단국대학교 창의융합제조공학과
  • 2단국대학교 화학공학과
Juseung Kim1, Eun Jin Jung1, Ji Hye Kim1, and Woo-Kul Lee1, 2, †
  • 1Department of Creative Convergent Manufacturing Engineering, Dankook University, Korea
  • 2Department of Chemical Engineering, Dankook University, 152, Jukjeon-ro, Suji-gu, Yongin-si, Gyeonggi-do 16890, Korea
E-mail:
초록
본 연구에서는 acetic acid와 NaOH를 활용하여 키토산(CHT) 지지체를 제조하였다. 또한 CHT 용액에 span80을 녹인 미네랄 오일(mineral oil) (MS) 용액을 혼합한 용액을 사용하여 제조한 지지체의 물리적 특성 변화에 관한 연구를 수행하였다. CHT/MS은 40/10과 60/10의 비율로 혼합하여 사용하였다. 순수한 CHT 용액으로만 제조한지지체는 대조군(control)으로 사용하였다. CHT/MS 혼합비율이 지지체의 표면 및 3차원 구조, 화학적 상태, 회복력, 기공도, 팽윤율 및 세포독성 등에 미치는 영향을 분석하였다. 분석결과에 의하면 CHT/MS 혼합용액을 사용하는 경우, control에 비해 미세한 기공 형성과 기공도, 지지체의 팽윤율이 증가하는 것을 확인하였다. 이러한 영향은 CHT/MS 60/10 지지체에서 더 현저하게 나타났다. 지지체에서의 조골세포 증식실험에서 MS 용액 혼합에 의한 지지체의 물리적 특성 변화는 세포독성을 유발시키지 않았다. 본 연구의 결과는 MS 용액을 활용하여 지지체의 세포적합성을 유지하면서 동시에 물리적 특성을 조절할 수 있다는 것을 확인하였다. 이 기술은 연조직 대체용 지지체 제조에도 활용할 수 있을 것으로 기대된다.
In this study, various chitosan (CHT) scaffolds were prepared using acetic acid and NaOH. In addition, we performed investigation on the changes in physical properties of the scaffolds prepared using a mixture of CHT solution and span 80-containing mineral oil (MS) solution. CHT/MS ratios were set at 40/10 and 60/10. The scaffold prepared using pure CHT solution was used as a control. We evaluated the effects of CHT/MS mixing ratio on the surface and 3D structure, chemical state, recovery, porosity, swelling, and cytotoxicity of the scaffold. We observed that the fine pore formation, porosity, and swelling of the prepared scaffold increased when MS solution was used. The behavior was more pronounced when CHT/MS 60/10 was used. In osteoblast proliferation assay, any cytotoxicity was not induced due to usage of MS solution. From the result obtained in this investigation, we noticed that the physical properties of the CHT scaffold can be manipulated by using MS solution while maintaining its cytocompatibility. We expect that this technique can be further utilized in the preparation of soft tissue substitutes.
Keywords:chitosan;scaffold;porosity;swelling;spectroscopy;osteoblast;cytotoxicity
  1. Langer R, Vacanti J, Science, 260, 920 (1993)
  2. Kim IY, Seo SJ, Moon HS, Yoo MK, Park IY, Kim BC, Cho CS, Biotechnol. Adv., 26, 1 (2008)
  3. Yoshimoto H, Shin YM, Terai H, Vacanti JP, Biomaterials, 24, 2077 (2003)
  4. Gautam S, Dinda AK, Mishra NC, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 33, 1228 (2013)
  5. Wu S, Liu X, Yeung KWK, Liu C, Yang X, Mater. Sci. Eng. R-Rep., 80, 1 (2014)
  6. Park KM, Joung YK, Park KD, Lee SY, Lee MC, Macromol. Res., 16(6), 517 (2008)
  7. Croisier F, Jerome C, Eur. Polym. J., 49, 780 (2013)
  8. Kavya KC, Jayakumar R, Nair S, Chennazhi KP, Int. J. Biol. Macromol., 59, 255 (2013)
  9. Okamoto M, John B, Prog. Polym. Sci, 38, 1487 (2013)
  10. Hutmacher DW, Biomaterials, 21, 2529 (2000)
  11. Freed LE, Marquis JC, Nohria A, Emmanual J, Mikos AC, Langer R, J. Biomed. Mater. Res., 27, 11 (1993)
  12. Lu L, Zhu XM, Valenzuela RG, Richard G, Currier BL, Yaszemski MJ, Clin. Orthop. Rel. Res, 391, 251 (2001)
  13. Mogosanu GD, Grumezescub AM, Int. J. Pharm., 463, 127 (2014)
  14. Cha J, Lee WB, Park CR, Cho YW, Ahn CH, Kwon IC, Macromol. Res., 14(5), 573 (2006)
  15. Park SA, Lee JB, Kim YE, Kim JE, Lee JH, Shin JW, Kwon IK, Kim WD, Macromol. Res., 22(8), 882 (2014)
  16. Nitya G, Nair GT, Mony U, Chennazhi KP, Nair SV, J. Mater. Sci. -Mater. Med., 23, 1749 (2012)
  17. Cardea S, Baldino L, Pisanti P, Reverchon E, J. Mater. Sci. -Mater. Med., 25, 355 (2014)
  18. Hu J, Sun X, Ma H, Xie C, Chen YE, Ma PX, Biomaterials, 31, 7971 (2010)
  19. Jin CZ, Choi BH, Park SR, Min BH, J. Biomed. Mater. Res., 92, 1567 (2010)
  20. Wang C, Cen L, Yin S, Liu Q, Liu W, Cao Y, Cui L, Biomaterials, 31, 621 (2010)
  21. Li J, Tao R, Wu W, Cao H, Xin J, Li J, Guo J, Jiang L, Gao C, Demetriou AA, Farkas DL, Li L, Stem Cells Dev., 19, 1427 (2010)
  22. Zhang Y, Yang F, Liu K, Shen H, Zhu Y, Zhang W, Liu W, Wang S, Cao Y, Zhou G, Biomaterials, 33, 2926 (2012)
  23. Yang B, Li X, Shi S, Kong X, Guo G, Huang M, Luo F, Wei Y, Zhao X, Qian Z, Carbohydr. Polym., 80, 860 (2010)
  24. Ji C, Annabi N, Khademhosseini A, Dehghani F, Acta Biomater., 7, 1653 (2011)
  25. Dhandayuthapani B, Krishnan UM, Sethuraman S, J. Biomed. Mater. Res., 94, 264 (2010)
  26. Wang CC, Yang KC, Lin KH, Liu HC, Lin FH, Biomaterials, 32, 7118 (2011)
  27. Sapir Y, Kryukov O, Cohen S, Biomaterials, 32, 1838 (2011)
  28. Swetha M, Sahithi K, Moorthi A, Srinivasan N, Ramasamy K, Selvamurugan N, Int. J. Biol. Macromol., 47, 1 (2010)
  29. Zhao W, Jin X, Cong Y, Liu YY, Fu J, J. Chem. Technol. Biotechnol., 88(3), 327 (2013)
  30. Kim HL, Jung GY, Yoon JH, Han JS, Park YJ, Kim DG, Zhang M, Kim DJ, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 54, 20 (2015)
  31. Wen S, Wang Z, Zheng X, Wang X, Mater. Lett., 186, 17 (2017)
  32. Baldino L, Cardea S, De Marco I, Reverchon E, J. Supercrit. Fluids, 90, 27 (2014)
  33. Ma L, Gao C, Mao Z, Zhou J, Shen J, Hu X, Han C, Biomaterials, 24, 4833 (2003)
  34. Bhattarai N, Gunn J, Zhang M, Adv. Drug Deliv. Rev., 62, 83 (2010)
  35. Esquerdo VM, Cadaval TRS, Dotto GL, Pinto LAA, J. Colloid Interface Sci., 424, 7 (2014)
  36. Annapoorna M, Kumar PTS, Lakshman LR, Lakshmanan VK, Nair SV, Jayakumar R, Carbohydr. Polym., 92, 1561 (2013)
  37. Li G, Zhang L, Wang C, Zhao X, Zhu C, Zheng Y, Wang Y, Zhao Y, Yang Y, Carbohydr. Polym., 101, 718 (2014)
  38. Park SJ, Kim MS, Yu SM, Gu BK, Kim JI, Kim CH, Macromol. Res., 20(4), 397 (2012)
  39. Liu Y, Ma L, Gao C, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 32, 361 (2012)
  40. Hsieh WC, Liau JJ, Li YJ, Int. J. Polym. Sci., 2015, 1 (2015)
  41. Zakhem E, Elbahrawy M, Orlando G, Bitar KN, Surgery, 158, 1598 (2015)
  42. Martinez A, Blancoa MD, Davidenko N, Cameron RE, Carbohydr. Polym., 132, 606 (2015)
  43. Liu Y, Ma L, Gao C, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 32, 2361 (2012)
  44. Han J, Zhou Z, Yin R, Yang D, Nie J, Biol. Macromol., 46, 199 (2010)
  45. Kim SE, Suh DH, Yun YP, Lee JY, Park KS, Chung JY, Lee DW, Mater. Sci.- Mater. Med., 23, 2739 (2012)
  46. Kuo SM, Chang SJ, Lin LC, Chen CJ, J. Appl. Polym. Sci., 89(14), 3897 (2003)
  47. Li KG, Wang Y, Miao ZC, Xu DY, Tang YF, Feng MF, Biotechnol. Lett., 26(11), 879 (2004)
  48. Nirmala R, Il BW, Navamathavan R, El-Newehy MH, Kim HY, Macromol. Res., 19(4), 345 (2011)