화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.14, No.5, 573-578, October, 2006
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Preparation and Characterization of Cisplatin-Incorporated Chitosan Hydrogels, Microparticles, and Nanoparticles
Jueun Cha, Won Bum Lee, Chong Rae Park†, Yong Woo Cho1, Cheol-Hee Ahn2, and Ick Chan Kwon3
  • Enviro-Polymers Design Lab., Hyperstructured Organic Materials Research Center (HOMRC),School of Materials Science and Engineering, Seoul National University, Seoul 151-742, Korea
  • 1Department of Chemical Engineering, Hanyang University, Ansan 426-791, Korea
  • 2Hyperstructured Organic Materials Research Center (HOMRC), School of Materials Science and Engineering, Seoul National University, Seoul 151-742, Korea
  • 3Biomedical Research Center, Korea Institute of Science and Technology, Seoul 136-791, Korea
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Three different, polymer-platinum conjugates (hydrogels, microparticles, and nanoparticles) were synthesized by complexation of cis-dichlorodiammineplatinum(II) (cisplatin) with partially succinylated glycol chitosan (PSGC). Succinic anhydride was used as a linker to introduce cisplatin to glycol chitosan (GC). Succinylation of GC was investigated systematically as a function of the molar ratio of succinic anhydride to glucosamine, the methanol content in the reaction media, and the reaction temperature. By controlling the reaction conditions, water-soluble, partially water-soluble, and hydrogel-forming PSGCs were synthesized, and then conjugated with cisplatin. The complexation of cisplatin with water-soluble PSGC via a ligand exchange reaction of platinum from chloride to the carboxylates induced the formation of nano-sized aggregates in aqueous media. The hydrodynamic diameters of PSGC/cisplatin complex nano-aggregates, as determined by light scattering, were 180-300 nm and the critical aggregation concentrations (CACs), as determined by a fluorescence technique using pyrene as a probe, were 20-30 μg/mL. The conjugation of cisplatin with partially water-soluble PSGC, i.e., borderline between water-soluble and water-insoluble PSGC, produced micro-sized particles <500 μm. Cisplatin-complexed PSGC hydrogels were prepared from water-insoluble PSGCs. All of the cisplatin-incorporated, polymer matrices released platinum in a sustained manner without any significant initial burst, suggesting that they may all be useful as slow release systems for cisplatin. The release rate of platinum increased with the morphology changes from hydrogel through microparticle to nanoparticle systems.
Keywords:chitosan;platinum;hydrogel;microparticle;nanoparticle;controlled drug release
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