화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.38, No.2, 156-163, March, 2014
Export Citation
하이드록시아파타이트/락타이드 글리콜라이드 공중합체 지지체 조성에 따른 염증 완화 효과
Inflammatory Responses to Hydroxyapatite/Poly(lactic-co-glycolic acid) Scaffolds with Variation of Compositions
장지은, 김혜민, 김형석, 전대연, 박찬흠1, 권순용2, 정진화2, †, 강길선†
  • 전북대학교 BIN 융합공학과, 고분자 나노공학과
  • 1한림대학교 의과대학 춘천성심병원 이비인후과
  • 2가톨릭대학교 의과대학 여의도성모병원 정형외과
Ji Eun Jang, Hye Min Kim, Hyeongseok Kim, Dae Yeon Jeon, Chan Hum Park1, Soon Yong Kwon2, Jin Wha Chung2, †, and Gilson Khang†
  • Dept. of BIN Fusion Tech., and Dept. of Polymer-Nano Sci. & Tech. and Polymer BIN Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin, Jeonju 561-756, Korea
  • 1Dept. of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, 1-1 Okcheon, Chuncheon, Gangwon 200-702, Korea
  • 2Dept. of Orthopedic Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, 62 Yeouido-dong, Yeongdeungpo-gu, Seoul 150-896, Korea
E-mail:,
초록
하이드록시아파타이트는 골 전도가 우수하고 생체 적합성이 우수하며 염증 반응을 일으키지 않아 임상에서 골이식재로 널리 사용되고 있다. 본 연구에서는 하이드록시아파타이트를 함유한 poly(lactic-co-glycolic acid) (PLGA) 지지체를 제조하였으며 생체 내/외의 실험을 통하여 골 이식재로서의 응용가능성을 평가하였다. 하이드록시아파타이트/PLGA 지지체는 0, 10, 20, 40 및 60 wt%의 함량으로 제조하였다. 기계적 특성을 알아보기 위하여 압축강도, SEM, FTIR을 측정하였으며 MTT, RT-PCR, FACS, 조직학적 염색(H&E, ED-1)을 실시하였다. 그 결과 하이드록시아파타이트를 함유한 PLGA 지지체에서 염증 반응이 감소하는 것을 확인할 수 있었으며 골 이식재로서의 가능성을 보여주었다.
Hydroxyapatite has osteoconductivity, biocompatibility and noninflammatory, and it has been used clinically as artificial bone. In this study, we prepared hydroxyapatite/poly(lactic-co-glycolic acid) (PLGA) scaffolds using 0, 10, 20, 40 and 60 wt% of hydroxyapatite. We analyzed compressive strength, SEM analysis and FTIR for mechanical property of 3D hydroxyapatite/PLGA scaffolds. For biocompatibility tests, cell proliferation and viability were measured via MTT assay and SEM. We analyzed RT-PCR, FACS, histology (H&E, ED-1) for anti-inflammatory effect. This study showed that hydroxyapatite hybrid scaffolds have low inflammatory reaction compared with the PLGA. This result has a potential for the application of artificial bone graft material.
Keywords:hydroxyapatite;inflammatory reaction;scaffold;artificial bone.
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