화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.38, No.2, 164-170, March, 2014
Export Citation
천연 고분자가 함유된 락타이드 글리콜라이드 공중합체 필름에서 슈반세포의 부착과 증식 거동 비교평가
Comparison between Poly(lactic-co-glycolic acid) Films Contained Natural Polymers on Adhesion and Proliferation of Schwann Cells
고현아, 장지은, 김형석, 박찬흠1, 권순용2, 정진화2, †, 강길선†
  • 전북대학교 BIN 융합공학과, 고분자 나노공학과
  • 1한림대학교 의과대학 춘천성심병원 이비인후과
  • 2가톨릭대학교 의과대학 여의도성모병원 정형외과
Hyun Ah Ko, Ji Eun Jang, Hyeongseok Kim, Chan Hum Park1, Soon Yong Kwon2, Jin Wha Chung2, †, and Gilson Khang†
  • Dept. of BIN Fusion Tech., and Dept. of Polymer-Nano Sci. & Tech. and Polymer BIN Research Center, Chonbuk National University, 567 Deokjin, Jeonju, Chonbuk 561-756, Korea
  • 1Dept. of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, 1-1 Okcheon, Chuncheon, Gangwon 200-702, Korea
  • 2Dept. of Orthopedic Surgery, Yeouido St. Mary’s Hospital, Catholic University of Korea, 62 Yeouido-dong, Yeongdeungpo-gu, Seoul 150-896, Korea
E-mail:,
초록
본 연구에서는 신경세포인 슈반세포(SC)의 증식에 가장 적합한 생체재료를 연구하였다. 락타이드 글리콜라이드 공중합체(PLGA)에 탈미네랄 골분(demineralized bone particle, DBP), 소장점막하조직(small intestine submucosa, SIS), 그리고 실크를 각각 20% 첨가하여, 용매 증발법으로 각각의 필름을 제조하고, SC세포의 부착과 증식을 확인하기 위해 MTT, SEM 그리고 RT-PCR 분석을 실시하였다. 또한 필름의 친수성을 확인하기 위해 접촉각을 측정하였다. 분석 결과, PLGA/DBP 20% 필름에서 높은 친수성을 보였으며, SC의 부착과 증식률이 다른 군에 비해 크게 증가한 것을 확인할 수 있었다. 따라서 PLGA/DBP 필름은 중추신경재생 재료로 활용할 수 있을 것으로 사료된다.
This study was designed to find an appropriate biomaterial to proliferate Schwann cell (SC). Poly(lactic-coglycolic acid) (PLGA) films mixed with demineralized bone particle (DBP), small intestine submucosa (SIS), and silk were fabricated by a solvent casting method. Analysis of MTT, SEM and RT-PCR were performed to confirm adhesion and proliferation of SC. Contact angle of films was assayed for hydrophilicity of films. We confirmed that PLGA/DBP 20% film showed higher hydrophilicity, promoted adhesion and proliferation of SC than other films. It was concluded that PLGA/DBP film can be applied for the scaffold biomaterials for the regeneration of central nerve system.
Keywords:schwann cell;demineralized bone particle (DBP);small intestine submucosa (SIS);silk;PLGA hybridized film.
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