Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures

Ujita M; Yamanaka M; Maeno Y; Yoshida K; Ohshio W; Ueno Y; Banno Y; Fujii H; Okumura H

Journal of Bioscience and Bioengineering, Vol.111, No.1, 22-25, 2011

DOI10.1016/j.jbiosc.2010.08.020 Export Citation

Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures

Ujita M, Yamanaka M, Maeno Y, Yoshida K, Ohshio W, Ueno Y, Banno Y, Fujii H, Okumura H

Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Silkworm trehalase;Hexahistidine tag;Baculovirus-silkworm expression system;Enzymatic activity;Oligosaccharide chains