Journal of Bioscience and Bioengineering, Vol.111, No.1, 19-21, 2011
Optical resolution of n-butyl D- and L-lactates using immobilized lipase catalyst
n-Butyl D- and L-lactates (BuDLa and BuLLa) were incubated with immobilized lipase. H-1-NMR showed that BuDLa reacted to oligomers, while BuLLa did not react. A mixture containing 90.4% of BuLLa and 9.6% of BuDla was incubated with the enzyme for 72 h, then distilled. The purity of BuLLa increased to 98.6%. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.