Reduced Inflammatory Responses to Poly(lactic-co-glycolic acid) by the Incorporation of Hydroxybenzyl Alcohol Releasing Polyoxalate

Sujin Kim; Yujung Lee; Hyunjin Park; Donghyun Hong; Gilson Khang; Dongwon Lee

Macromolecular Research, Vol.19, No.12, 1242-1249, December, 2011

DOI10.1007/s13233-011-1215-z Export Citation

Reduced Inflammatory Responses to Poly(lactic-co-glycolic acid) by the Incorporation of Hydroxybenzyl Alcohol Releasing Polyoxalate

Sujin Kim¹, Yujung Lee¹, Hyunjin Park¹, Donghyun Hong², Gilson Khang¹, and Dongwon Lee^{1, 2, †}

¹Polymer Fusion Research Center, Department of Polymer Nano Science and Technology, Chonbuk National University, Jeonbuk, 561-756, Korea
²Department of BIN Fusion Technology, Chonbuk National University, Jeonbuk, 561-756, Korea

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Poly(lactic-co-glycolic acid) (PLGA) has been widely used in biomedical and pharmaceutical applications because of its satisfactory biocompatibility and biodegradation profile. However, its applications for tissue engineering scaffolds have been plagued by inflammatory responses that can lead to fibrotic encapsulation. p-Hydroxybenzyl alcohol (HBA) is a phenolic compound in herbal agents that exerts antioxidant and anti-inflammatory activities. In this work, we developed PLGA blend films containing HBA-incorporated peroxalate polymer (HPOX) and investigated the effects of HPOX on the local inflammatory responses to PLGA in vitro and in vivo. The PLGA/HPOX blend films had no adverse effects on cell viability and elicited a significantly lower production of pro-inflammatory mediators such as tumor necrosis factor-α and reactive oxygen species than PLGA films. We also found that PLGA/HPOX blend films implanted into a rat had significantly diminished inflammatory responses. PLGA/HPOX blends appear to be a promising approach for tissue engineering applications due to their excellent biocompatibility and anti-inflammatory effects.

Keywords:biodegradation;inflammation;PLGA;TNF-α;scaffold.

[References]

Zhang XH, Reagan MR, Kaplan DL, Adv. Drug Deliv. Rev., 61, 988 (2009)
Thevenot PT, Nair AM, Shen JH, Lotfi P, Ko CY, Tang LP, Biomaterials., 31, 3997 (2010)
Acharya C, Kumary TV, Ghosh SK, Kundu SC, J.Biomater. Sci. Polym. Ed., 20, 543 (2009)
Silva M, Cyster LA, Barry JJA, Yang XB, Oreffo ROC, Grant DM, Scotchford CA, Howdle SM, Shakesheff KM, Rose F, Biomaterials., 27, 5909 (2006)
Cao WL, Wang AJ, Jing DH, Gong YD, Zhao NM, Zhang XF, J. Biomater. Sci. Polym. Ed., 16, 1379 (2005)
Ho ML, Fu YC, Wang GJ, Chen HT, Chang JK, Tsai TH, Wang CK, J. Control. Release., 128, 142 (2008)
Xie SJ, Zhu Q, Wang B, Gu HJ, Liu W, Cui L, Cen LA, Cao YL, Biomaterials., 31, 5100 (2010)
Yoon SJ, Kim SH, Ha HJ, Ko YK, So JW, Kim MS, Yang YI, Khang G, Rhee JM, Lee HB, Tissue Eng. Part A., 14, 539 (2008)
Kim MS, Ahn HH, Shin YN, Cho MH, Khang G, Lee HB, Biomaterials., 28, 5137 (2007)
Liu JK, Mori A, Neuropharmacology., 32, 659 (1993)
Lim EJ, Kang HJ, Jung HJ, Park EH, J. Pharm.Pharmacol., 59, 1235 (2007)
Park H, Kim S, Kim S, Song Y, Seung K, Hong D, Khang G, Lee D, Biomacromolecules, 11(8), 2103 (2010)
Lu SZ, Wang XQ, Lu Q, Zhang XH, Kluge JA, Uppal N, Omenetto F, Kaplan DL, Biomacromolecules, 11(1), 143 (2010)
Serrano MC, Pagani R, Vallet-Regi M, Pena J, Ramila A, Izquierdo I, Portoles MT, Biomaterials., 25, 5603 (2004)
Yi F, Lu JW, Guo ZX, Yu J, J. Biomater. Sci. Polym.Ed., 17, 1015 (2006)
Kim S, Park H, Song Y, Hong D, Kim O, Jo E, Khang G, Lee D, Biomaterials., 32, 3021 (2011)
Panilaitis B, Altman GH, Chen JS, Jin HJ, Karageorgiou V, Kaplan DL, Biomaterials., 24, 3079 (2003)
Yim ES, Zhao B, Myung D, Kourtis LC, Frank CW, Carter D, Smith RL, Goodman SB, J. Biomed.Mater. Res. A., 91A, 894 (2009)
Yang YW, Hsu PYJ, Biomaterials., 29, 2516 (2008)
Serrano M, Pagani R, Manzano M, Comas JV, Portoles MT, Biomaterials., 27, 4706 (2006)
Robinson JP, Bruner LH, Bassoe CF, Hudson JL, Ward PA, Phan SH, J. Leukoc. Biol., 43, 304 (1988)
Athanasiou KA, Niederauer GG, Agrawal CM, Biomaterials., 17, 93 (1996)
Baumann MD, Kang CE, Tator CH, Schoichet MS, Biomaterials., 31, 7631 (2010)
Wu ACK, Grondahl L, Jack KS, Foo MX, Trau M, Hume DA, Cassady AI, Biomaterials., 27, 4715 (2006)
Cadenas E, Mol. Aspects Med., 25, 17 (2004)
Ozmen I, Naziroglu M, Okutan R, Cell Biochem. Funct., 24, 275 (2006)
Rhodes NP, Biomaterials., 28, 5131 (2007)
Tracey KJ, Cerami A, Annu. Rev. Med., 45, 491 (1994)
Wu Z, Zhang J, Nakanishi H, J. Neuroimmunol., 167, 90 (2005)
Numata M, Suzuki S, Miyazawa N, Miyashita A, Nagashima Y, Inoue S, Kaneko T, Okubo T, J. Immunol., 160, 3031 (1998)
Azad N, Rojanasakul Y, Vallyathan V, J. Toxicol. Environ.Health. B: Crit. Rev., 11, 1 (2008)

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[2] Thevenot PT, Nair AM, Shen JH, Lotfi P, Ko CY, Tang LP, Biomaterials., 31, 3997 (2010)

[3] Acharya C, Kumary TV, Ghosh SK, Kundu SC, J.Biomater. Sci. Polym. Ed., 20, 543 (2009)

[4] Silva M, Cyster LA, Barry JJA, Yang XB, Oreffo ROC, Grant DM, Scotchford CA, Howdle SM, Shakesheff KM, Rose F, Biomaterials., 27, 5909 (2006)

[5] Cao WL, Wang AJ, Jing DH, Gong YD, Zhao NM, Zhang XF, J. Biomater. Sci. Polym. Ed., 16, 1379 (2005)

[6] Ho ML, Fu YC, Wang GJ, Chen HT, Chang JK, Tsai TH, Wang CK, J. Control. Release., 128, 142 (2008)

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[8] Yoon SJ, Kim SH, Ha HJ, Ko YK, So JW, Kim MS, Yang YI, Khang G, Rhee JM, Lee HB, Tissue Eng. Part A., 14, 539 (2008)

[9] Kim MS, Ahn HH, Shin YN, Cho MH, Khang G, Lee HB, Biomaterials., 28, 5137 (2007)

[10] Liu JK, Mori A, Neuropharmacology., 32, 659 (1993)

[11] Lim EJ, Kang HJ, Jung HJ, Park EH, J. Pharm.Pharmacol., 59, 1235 (2007)

[12] Park H, Kim S, Kim S, Song Y, Seung K, Hong D, Khang G, Lee D, Biomacromolecules, 11(8), 2103 (2010)

[13] Lu SZ, Wang XQ, Lu Q, Zhang XH, Kluge JA, Uppal N, Omenetto F, Kaplan DL, Biomacromolecules, 11(1), 143 (2010)

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[15] Yi F, Lu JW, Guo ZX, Yu J, J. Biomater. Sci. Polym.Ed., 17, 1015 (2006)

[16] Kim S, Park H, Song Y, Hong D, Kim O, Jo E, Khang G, Lee D, Biomaterials., 32, 3021 (2011)

[17] Panilaitis B, Altman GH, Chen JS, Jin HJ, Karageorgiou V, Kaplan DL, Biomaterials., 24, 3079 (2003)

[18] Yim ES, Zhao B, Myung D, Kourtis LC, Frank CW, Carter D, Smith RL, Goodman SB, J. Biomed.Mater. Res. A., 91A, 894 (2009)

[19] Yang YW, Hsu PYJ, Biomaterials., 29, 2516 (2008)

[20] Serrano M, Pagani R, Manzano M, Comas JV, Portoles MT, Biomaterials., 27, 4706 (2006)

[21] Robinson JP, Bruner LH, Bassoe CF, Hudson JL, Ward PA, Phan SH, J. Leukoc. Biol., 43, 304 (1988)

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[24] Wu ACK, Grondahl L, Jack KS, Foo MX, Trau M, Hume DA, Cassady AI, Biomaterials., 27, 4715 (2006)

[25] Cadenas E, Mol. Aspects Med., 25, 17 (2004)

[26] Ozmen I, Naziroglu M, Okutan R, Cell Biochem. Funct., 24, 275 (2006)

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[28] Tracey KJ, Cerami A, Annu. Rev. Med., 45, 491 (1994)

[29] Wu Z, Zhang J, Nakanishi H, J. Neuroimmunol., 167, 90 (2005)

[30] Numata M, Suzuki S, Miyazawa N, Miyashita A, Nagashima Y, Inoue S, Kaneko T, Okubo T, J. Immunol., 160, 3031 (1998)

[31] Azad N, Rojanasakul Y, Vallyathan V, J. Toxicol. Environ.Health. B: Crit. Rev., 11, 1 (2008)