Intracellular Uptake of Magnetite Nanoparticles Conjugated with RGDS-Peptide

Zhi-Cai Xing; Min-Jeong Park; Seung-Jin Han; Inn-Kyu Kang; Moon-Jeong Choi; Byung-Heon Lee; Yongmin Chang

Macromolecular Research, Vol.19, No.9, 897-903, September, 2011

DOI10.1007/s13233-011-0902-0 Export Citation

Intracellular Uptake of Magnetite Nanoparticles Conjugated with RGDS-Peptide

Zhi-Cai Xing, Min-Jeong Park, Seung-Jin Han, Inn-Kyu Kang^†, Moon-Jeong Choi¹, Byung-Heon Lee², and Yongmin Chang³

Department of Polymer Science and Engineering, Kyungpook National University, Daegu, 702-701, Korea
¹Medical and Biological Engineering, Kyungpook National University, Daegu, 702-701, Korea
²Department of Biochemistry, School of Medicine, Kyungpook National University, Daegu, 700-422, Korea
³Department of Diagnostic Radiology, Kyungpook National University, Daegu, 700-422, Korea

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Peptides containing the Arg-Gly-Asp (RGD) motif inhibit cell adhesion and exhibit a range of other biological effects including anticoagulant and antimetastatic activities. This study examined the anchorage independent effects of an RGD-containing peptide, Arg-Gly-Asp-Ser (RGDS), on NIH 3T3 fibroblasts. Magnetite nanoparticles were prepared by the coprecipitation of ferrous iron (Fe2+) and ferric iron (Fe3+) with NH4OH and dextran (DNPs). The aminosilane agent of 3-aminopropyltriethoxysilane (A) is considered to be a candidate for modification of the surface of magnetite nanoparticles (ADNPs). The amino-functionalized magnetite nanoparticles were activated using the glutaraldehyde (G) method (GADNPs). Subsequently, RGD or RGDS peptides were immobilized on the glutaraldehyde-modified magnetite nanoparticles (RGADNPs or RSGADNPs). The RSGADNPs were evaluated using Fourier-transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). The biological responses of the RSGADNPs were evaluated using NIH 3T3 fibroblast cells. Inductively coupled plasma mass spectrophotometry (ICP-MS) and Prussian blue staining showed that the RSGADNPs were internalized into the NIH 3T3 cells compared to the RGADNPs. These results suggest that RSGADNP may find ever-growing applications in biological labels and detection or contrast agents in life science and medical diagnostics.

Keywords:magnetite nanoparticles;RGD peptide;RGDS peptide;immobilization;internalization.

[References]

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[4] Weissleder R, Moore A, Mahmood U, Bhorade R, Benveniste H, Nat. Med., 6, 351 (2000)

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[10] O'Brien S, Brus L, Murray CB, J. Am. Chem. Soc., 123(48), 12085 (2001)

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[12] Wang X, Zhuang J, Peng Q, Li Y, Nature, 437, 121 (2005)

[13] El-Boubbou K, Zhu DC, Vasileiou C, Borhan B, Prosperi D, Li W, Huang XF, J. Am. Chem. Soc., 132(12), 4490 (2010)

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[18] Gupta AK, Curtis ASG, Proceedings of the 30th Annual Symposium of Controlled Release of Bioactive Materials, 30, 788 (2003)

[19] Gupta AK, Berry C, Gupta M, Curtis A, IEEE Trans. Nanobiosci., 2, 256 (2003)

[20] Kaneo Y, Tanaka T, Nakano T, Yamaguchi Y, J. Control. Release, 70, 365 (2001)

[21] Debelle L, Tamburro AM, Int. J. Biochem. Cell Biol., 31, 261 (1999)

[22] Kamruzzaman Selim KM, Lee JH, Kim SJ, Xing Z, Kang IK, Chang Y, Guo H, Macromol. Res., 14(6), 646 (2006)

[23] Selim KKM, Park MJ, Kim HM, Kang IK, Key Eng. Mater., 342-333, 793 (2007)

[24] Shi Z, Neoh KG, Kang ET, Shuter B, Wang SC, Poh C, Wang W, Appl. Mater. Interfaces, 1, 328 (2009)

[25] Molday RS, MacKenzie D, J. Immunol. Methods, 52, 353 (1982)

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[29] Antony AC, Blood, 79, 2807 (1992)