화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.19, No.5, 487-494, May, 2011
DOI10.1007/s13233-011-0505-9  Export Citation
Heparin-Based Self-Assembled Nanoparticles for Photodynamic Therapy
Li Li, Hyun Tae Moon1, Jin-Young Park2, Yu Jung Heo2, Yongdoo Choi2, Thanh Huyen Tran, Yong-kyu Lee3, So Yeon Kim4, †, and Kang Moo Huh†
  • Department of Polymer Science and Engineering, Chungnam National University, Daejeon, 305-764, Korea
  • 1Mediplex Corp., Seoul, 135-729, Korea
  • 2Molecular Imaging & Therapy branch, National Cancer Center, Goyang, 410-769, Korea
  • 3Department of Chemical and Biological Engineering, Chungju National University, Chungju, 380-702, Korea
  • 4Department of Chemical Engineering Education, Chungnam National University, Daejeon, 305-764, Korea
E-mail:,
Novel heparin-based self-assembled nanoparticles were developed for photodynamic cancer therapy. A heparin-poly(β-benzyl-L-aspartate) (HP) amphiphilic copolymer was synthesized for effective delivery of a hydrophobic photosensitizer, pheophorbide a (Pheo). The anti-coagulant activity of the HP copolymer decreased significantly, as measured by an anti-FXa chromogenic assay. Pheo was effectively incorporated into the HP nanoparticles using a dialysis technique. A good drug-loading content was obtained by altering the composition of the HP copolymer and Pheo/HP feed ratio. The Pheo-loaded HP nanoparticles had an average diameter of 117-189 nm, a negatively charged surface, and a sustained drug release pattern. These properties may allow them to passively target the tumor site through an enhanced permeability and retention (EPR) effect. Furthermore, the Pheo-loaded HP nanoparticles demonstrated marked photocytotoxicity and minimal darktoxic without a light treatment. The 6HP-Pheo15 nanoparticles were internalized into SCC7 cancer cells and damaged the cells after a light treatment. This suggests that HP nanoparticles have potential as an effective delivery system for clinical PDT.
Keywords:self-assembled nanoparticles;photodynamic therapy;heparin;drug delivery.
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