Depigmenting effect of Sterculia lynchnophera on B16F10 melanoma and C57BL/6 melan-a cells

Uyen Do Phuong Lam; Dung Nguyen Hoang; Hyang-Bok Lee; Bora Kim; Joo-Dong Lee; Jeong-Hyun Shin; Eun-Ki Kim

Korean Journal of Chemical Engineering, Vol.28, No.4, 1074-1077, April, 2011

DOI10.1007/s11814-010-0484-0 Export Citation

Depigmenting effect of Sterculia lynchnophera on B16F10 melanoma and C57BL/6 melan-a cells

Uyen Do Phuong Lam, Dung Nguyen Hoang, Hyang-Bok Lee, Bora Kim¹, Joo-Dong Lee¹, Jeong-Hyun Shin², and Eun-Ki Kim^†

Department of Biological Engineering, National Research Lab of Skin Bioactive Material, Inha University, Incheon 402-751, Korea
¹Cosmetic Research Center, Enprani Co., Ltd., Incheon 400-103, Korea
²Department of Dermatology, College of Medicine, Inha University, Incheon 402-751, Korea

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To develop a novel skin depigmenting agent from natural sources, the inhibition of melanogenesis by the Chinese herb, Sterculia lynchnophera (SL), was evaluated. Treatment of B16F10 melanoma cells and melan-a cells with SL exhibited a 32.9% and 68.2% inhibition of melanin synthesis without cytotoxicity at a concentration of 200μg/ml, respectively. This herb possessed a high free radical scavenging activity with IC50=11.02 μM. The methanol extract of SL slightly inhibited in vitro mushroom tyrosinase activity (23.4% at a concentration of 200 μg/ml) and had a significant inhibitory effect on cellular tyrosinase activivity (48.65% and 88.56% inhibition at the concentration 200μg/ml in B16F10 cells and C57BL/6 melan-a cells, respectively). From the western blotting results, SL inhibited the expression of tyrosinase and tyrosinase related protein 1 (TRP-1). Taken together, we suggest that SL may be a safe and effective depigmentation agent.

Keywords:Depigmenting;Melanin;Melanogenesis;Tyrosinase

[References]

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Barsh GS, Am. J. Hum. Genet., 57, 743 (1995)
Brilliant M, Gardner J, J. Invest. Dermatol., 117, 386 (2001)
Ferguson CA, Kidson SH, Pigment. Cell. Res., 10, 127 (1997)
Slominski A, Tobin DJ, Shibahara S, Wortsman J, Physiol. Rev., 84, 1155 (2004)
Riley PA, Pigment. Cell. Res., 16, 548 (2003)
Baek SH, Kim DH, Lee CY, Kho YH, Lee CH, J. Microbiol. Biotechnol., 16, 667 (2006)
Kim WG, Ryoo IJ, Park SH, Kim DS, Lee SK, Park KC, Yoo ID, J. Microbiol. Biotechnol., 15, 891 (2005)
Lee SH, Choi SY, Kim HC, Hwang JS, Lee BG, Gao JJ, Kim SY, Biol. Pharm. Bull., 25, 1045 (2002)
Lim YH, Kim IH, Seo JJ, Kim JK, J. Microbiol. Biotechnol., 16, 1977 (2006)
Masamoto Y, Murata Y, Baba K, Shimoishi Y, Tada M, Takahata K, Biol. Pharm. Bull., 27, 422 (2004)
Miyazawa M, Oshima T, Koshio K, Itsuzaki Y, Anzai J, J. Agric. Food Chem., 51, 6953 (2003)
Parvez S, Kang M, Chung HS, Cho C, Hong MC, Shin MK, Bae HS, Phytother. Res., 20, 921 (2006)
Petit L, Pierard GE, Int. J. Cosmet. Sci., 25, 169 (2003)
Solano F, Briganti S, Picardo M, Ghanem G, Pigment. Cell. Res., 19, 549 (2006)
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Chen JM, Li WK, Liu HL, Shen YY, Yu MQ, Zhongyaocai., 18, 567 (1995)
Chen J, Cao P, Song H, Zhongguo Zhong Yao Za Zhi., 21, 39 (1996)
Wang RF, Yang XW, Ma CM, Shang MY, Liang JY, Wang W, Cai SQ, Shoyama Y, Phytochemistry., 63, 475 (2003)
Nguyen DH, Nguyen DTM, La LH, Yang SH, Lee HB, Kim HJ, Shin JH, Kim DM, Kim EK, Korean J. Chem. Eng., 24(5), 827 (2007)
Hall AM, Orlow SJ, Pigment. Cell. Res., 18, 122 (2005)
Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, Kubota Y, Pigment. Cell. Res., 16, 629 (2003)

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[3] Brilliant M, Gardner J, J. Invest. Dermatol., 117, 386 (2001)

[4] Ferguson CA, Kidson SH, Pigment. Cell. Res., 10, 127 (1997)

[5] Slominski A, Tobin DJ, Shibahara S, Wortsman J, Physiol. Rev., 84, 1155 (2004)

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[9] Lee SH, Choi SY, Kim HC, Hwang JS, Lee BG, Gao JJ, Kim SY, Biol. Pharm. Bull., 25, 1045 (2002)

[10] Lim YH, Kim IH, Seo JJ, Kim JK, J. Microbiol. Biotechnol., 16, 1977 (2006)

[11] Masamoto Y, Murata Y, Baba K, Shimoishi Y, Tada M, Takahata K, Biol. Pharm. Bull., 27, 422 (2004)

[12] Miyazawa M, Oshima T, Koshio K, Itsuzaki Y, Anzai J, J. Agric. Food Chem., 51, 6953 (2003)

[13] Parvez S, Kang M, Chung HS, Cho C, Hong MC, Shin MK, Bae HS, Phytother. Res., 20, 921 (2006)

[14] Petit L, Pierard GE, Int. J. Cosmet. Sci., 25, 169 (2003)

[15] Solano F, Briganti S, Picardo M, Ghanem G, Pigment. Cell. Res., 19, 549 (2006)

[16] Yoshimura M, Watanabe Y, Kasai K, Yamakoshi J, Koga T, Biosci. Biotechnol. Biochem., 69, 2368 (2005)

[17] Zhong S, Wu Y, Ahn SM, Zhao J, Wang K, Yang S, Yeon JH, Zhu X, Biol. Pharm. Bull., 29, 1947 (2006)

[18] Wang RF, Yang XW, Ma CM, Shang MY, Yang S, Wang MC, Cai SQ, Zhongguo Zhong Yao Za Zhi., 28, 533 (2003)

[19] Chen JM, Li WK, Liu HL, Shen YY, Yu MQ, Zhongyaocai., 18, 567 (1995)

[20] Chen J, Cao P, Song H, Zhongguo Zhong Yao Za Zhi., 21, 39 (1996)

[21] Wang RF, Yang XW, Ma CM, Shang MY, Liang JY, Wang W, Cai SQ, Shoyama Y, Phytochemistry., 63, 475 (2003)

[22] Nguyen DH, Nguyen DTM, La LH, Yang SH, Lee HB, Kim HJ, Shin JH, Kim DM, Kim EK, Korean J. Chem. Eng., 24(5), 827 (2007)

[23] Hall AM, Orlow SJ, Pigment. Cell. Res., 18, 122 (2005)

[24] Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, Kubota Y, Pigment. Cell. Res., 16, 629 (2003)