ANALYSIS OF CULTURE FLUORESCENCE BY A FIBER-OPTIC SENSOR IN NI	COTIANA TABACUM PLANT CELL CULTURE

Choi JW; Park JH; Lee SC; Kim DI; Lee WH

Korean Journal of Chemical Engineering, Vol.12, No.5, 528-534, November, 1995

ANALYSIS OF CULTURE FLUORESCENCE BY A FIBER-OPTIC SENSOR IN NI COTIANA TABACUM PLANT CELL CULTURE

Choi JW, Park JH, Lee SC, Kim DI, Lee WH

The on-line sensing of viable cell weight in plant cell culture process is applied to analysis and control of process. The fiber-optic fluorescence sensor was constructed to measure the NADH-dependent fluorescence in Nicotiana tabacum plant cell culture and the analysis of fluorescence signal was done to be correlated with the viable cell weight. The structured kinetic model for cell growth was proposed to estimate the theoretical viable cell weight. The dimensional analysis was proposed for the interpretation of fluorescence signal, in which the path length, the inner filter effect and the hydrodynamic conditions were considered as the key factors on fluorescence signal. The dimensional analysis and empirical correlation of fluorescence signal to viable cell weight was applied to the interpretation of the detected fluorescence signal during cultivation. The proposed interpretation of fluorescence signal using dimensional analysis was well correlated with the viable cell weight estimated by the structured kinetic model as well as by empirical correlation.

Keywords:Nicotiana tabacum;Fiber-Optic Sensor;NADH Fluorescence;Dimensional Analysis;Structured Kinetic Model

[References]

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[2] Anders KD, Muller W, Scheper T, Buckmann AF, "Use of Fluorescence Sensors: I. For the Monitoring of Immobilized Cell Culture Fluorescence II. As Optical Biosensors," Biochemical Engineering VI, Goldstein, W., DiBiasico, D. and Pedersen, H., eds., New York Academy of Science, New York (1990)

[3] Baeyens W, DeKeukeleire D, Korkidis K, "Luminescence Techniques in Chemical and Biochemical Analysis," Marcel Dekker, Inc., New York (1991)

[4] Coppella SJ, Rao G, Biotechnol. Prog., 6 (1990)

[5] Dixon RA, "Isolation and Maintenance of Callus and Cell Suspension Cultures," Plant Cell Culture: A Practical Approach, Dixon, R.A., ed., IRL Press, Oxford (1986)

[6] Duysens LNM, Amesz J, Biochim. Biophys. Acta, 24, 19 (1957)

[7] Groom CA, Luong JHT, Mulchandani A, J. Biotechnol., 8, 271 (1988)

[8] Humphrey AE, Brown K, Horvath J, Semerjian H, "The Use of Fluorometry in Monitoring and Control of Cell Cultures," Bioproducts and Bioprocesses, Fiechter, A., Okada, H. and Tanner, R.D., eds., Springer-Verlag, Berlin (1989)

[9] Junker BH, Wang DIC, Hatton TA, Biotechnol. Bioeng., 32, 55 (1988)

[10] Li JK, Humphrey AE, Biotechnol. Lett., 11, 177 (1989)

[11] Li JK, Asali EC, Humphrey AE, Horvath JJ, Biotechnol. Prog., 7, 21 (1991)

[12] Metzler CM, Elfring GL, McEwen AJ, Biometrics, 30, 562 (1974)

[13] Meyer C, Beyeler W, Biotechnol. Bioeng., 26, 916 (1984)

[14] Moser A, "Bioprocess Technology; Kinetics and Reactors," Springer-Verlag, New York (1988)

[15] Peck MW, Chynoweth DP, Biotechnol. Lett., 12, 17 (1990)

[16] Reardon KF, Scheper T, Bailey JE, Biotechnol. Prog., 3, 153 (1987)

[17] Reardon KF, Bailey JE, Biotechnol. Prog., 5, 144 (1989)

[18] Ristroph DL, Watteeuw GMW, Armiger B, Humphrey AE, J. Ferment. Technol., 55, 599 (1977)

[19] Walker CC, Dhurjati P, Biotechnol. Bioeng., 33, 500 (1989)

[20] Wang NS, Simmons MB, Biotechnol. Bioeng., 38, 1292 (1991)