CHARACTERISTICS OF CELLULASE MODIFIED WITH AMPHIPHILIC COPOLYMER IN ORGANIC SOLVENT

Park JW

Korean Journal of Chemical Engineering, Vol.12, No.5, 523-527, November, 1995

Characteristics of modified cellulases in organic solvents were studied. Cellulases modified with amphiphilic copolymer of polyoxyalkylene (POA)-derivative and maleic acid anhydride (MAA). Amino groups of the cellulase molecule were easily coupled with the MAA functional group of the copolymer. At the maximum degree of modification (DM) of 55%, the activity of modified cellulase retained more than 80% of the unmodified native cellulase activity. The modified cellulase using AKM-1511 with DM greater than 40% was found to be more than 90% soluble in aqueous solution of acetone and ethanol, leaving the native cellulase and impurities in the fermentation broth with the residue. Modified cellulase showed excellent stability against water-insoluble solvent. Moreover, cellulase modified with hydrophobic copolymer, which consists of ethylene oxide (EO) and propylene oxide (PO), could be dissolved in these solvents.

Keywords:Modified Cellulase;Amphiphilic Copolymer;Stability;Solubility in Organic Solvent

[References]

Ajima A, Inada Y, Matsushima A, Saito T, Takahashi K, Yoshimoto T, Trends Biotechnol., 4, 190 (1986)
Akehata T, Kajiuchi T, Park JW, Takahata Y, Biotechnol. Bioeng., 39, 117 (1992)
Albertsson PA, Persson I, Tjerneld F, Biotechnol. Bioeng., 15, 419 (1985)
Andreotti R, Mandels M, Roche C, Biotechnol. Bioeng., 6, 21 (1976)
Butler PJG, Harris J, Hartley I, Biochem. J., 112, 679 (1969)
Castanon M, Wilke CR, Biotechnol. Bioeng., 23, 1365 (1981)
Clanet M, Durand H, Tiraby G, Biotechnol. Bioeng., 32, 930 (1988)
Emert GH, Rivers DB, Biotechnol. Bioeng., 31, 278 (1988)
Habeeb AFSF, Anal. Biotech., 14, 328 (1966)
Harono Y, Ooshima H, Sakata M, Biotechnol. Bioeng., 28, 1727 (1986)
Inada Y, Kodera Y, Nishio T, Saito Y, Takahashi T, Yoshimoto Y, Biotechnol. Lett., 9, 187 (1987)
Moon HY, Kim SY, Park JW, Kajiuchi T, Korean J. Chem. Eng., 10(4), 235 (1993)
Kajiuchi T, Moon HY, Park JW, J. Chem. Eng. Jpn., 26, 28 (1993)
Kajiuchi T, Park JW, J. Chem. Eng. Jpn., 25, 202 (1992)
Miller GL, Anal. Chem., 31, 426 (1959)
Sinitsyn AP, Appl. Biochem. Biotechnol., 8, 25 (1983)

[Referenced By]

[1] Ajima A, Inada Y, Matsushima A, Saito T, Takahashi K, Yoshimoto T, Trends Biotechnol., 4, 190 (1986)

[2] Akehata T, Kajiuchi T, Park JW, Takahata Y, Biotechnol. Bioeng., 39, 117 (1992)

[3] Albertsson PA, Persson I, Tjerneld F, Biotechnol. Bioeng., 15, 419 (1985)

[4] Andreotti R, Mandels M, Roche C, Biotechnol. Bioeng., 6, 21 (1976)

[5] Butler PJG, Harris J, Hartley I, Biochem. J., 112, 679 (1969)

[6] Castanon M, Wilke CR, Biotechnol. Bioeng., 23, 1365 (1981)

[7] Clanet M, Durand H, Tiraby G, Biotechnol. Bioeng., 32, 930 (1988)

[8] Emert GH, Rivers DB, Biotechnol. Bioeng., 31, 278 (1988)

[9] Habeeb AFSF, Anal. Biotech., 14, 328 (1966)

[10] Harono Y, Ooshima H, Sakata M, Biotechnol. Bioeng., 28, 1727 (1986)

[11] Inada Y, Kodera Y, Nishio T, Saito Y, Takahashi T, Yoshimoto Y, Biotechnol. Lett., 9, 187 (1987)

[12] Moon HY, Kim SY, Park JW, Kajiuchi T, Korean J. Chem. Eng., 10(4), 235 (1993)

[13] Kajiuchi T, Moon HY, Park JW, J. Chem. Eng. Jpn., 26, 28 (1993)

[14] Kajiuchi T, Park JW, J. Chem. Eng. Jpn., 25, 202 (1992)

[15] Miller GL, Anal. Chem., 31, 426 (1959)

[16] Sinitsyn AP, Appl. Biochem. Biotechnol., 8, 25 (1983)