An artificial nanozyme model was developed by the supramolecular complexation of a beta-cyclodextrin-modified gold nanoparticle and metal catalytic centers. The cyclodextrin-based monolayer was first constructed on the surface of gold nanoparticle by using the thiol modified cyclodextrin, subsequently the cyclodextrin-modified gold nanoparticle was utilized as a backbone to install metal catalytic centers by supramolecular assembly of the copper complex of triethylnetetramine-adamantane and beta-cyclodextrin receptors immobilized on the surface of gold nanospheres via hydrophobic interaction. The catalytic behaviors of beta-cyclodextrin-modified gold nanoparticles with adjacent multi-metal catalytic centers were investigated as an esterase mimic. Strong hydrolase activities for catalyzing the cleavage of an active ester 4,4'-dinitrodiphenyl carbonate (DNDPC) were observed. A detailed kinetic study on nanozyme-catalyzed hydrolysis of ester DNDPC has been described.