Two series of Cu-Ce-O and Cu-Co-Ce-O catalysts were prepared by co-precipitation method. The prepared catalysts were characterized by XRD, IR, TPR, XPS, BET and ICP-AES. The catalytic activities of the catalysts for low-temperature CO oxidation were evaluated through a microreactor-GC system. TPR results indicate that the addition of cobalt to the Cu-Ce-O can increase the dispersion of copper oxide, and the interaction between cobalt and copper can enhance the reducibility of each other. XPS analysis show that Ce4+, Cu2+, along with Co3O4, are present on the surface of Cu0.4Co0.6Ce4 catalyst. The Co/Cu atomic ratio and the calcination temperature have significant effect on the activities of the catalysts. Compared with Cu1Ce4 catalyst, the Cu0.4Co0.6Ce4 catalyst has better activity and thermal stability.