Characterization of a carbon composite electrode for an electrochemical immunosensor

Eun Chan Cho; Byong Ok Jang; Eui Jung Kim; Kee-Kahb Koo

Korean Journal of Chemical Engineering, Vol.25, No.3, 548-552, May, 2008

DOI10.1007/s11814-008-0092-4 Export Citation

Characterization of a carbon composite electrode for an electrochemical immunosensor

Eun Chan Cho, Byong Ok Jang, Eui Jung Kim¹, and Kee-Kahb Koo^†

Department of Chemical and Biomolecular Engineering and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Korea
¹Department of Chemical Engineering, University of Ulsan, Ulsan 680-749, Korea

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A bioactive platform with a carbon composite electrode was developed for rapid detection of Escherichia coli O157:H7. The porous carbon composite electrode was prepared by a sol-gel method with a mixture of graphite powder and tetraethyl orthosilicate/ethanol. Escherichia coli O157:H7 antibodies were physically adsorbed onto the carbon composite electrode. Direct measurements by cyclic voltammetry and electrochemical impedance spectroscopy in the presence of [Fe(CN)6]^3-/4- as a redox probe showed that the immobilization of antibodies onto the carbon composite electrode surface and the binding of Escherichia coli O157:H7 cells with antibodies systematically increased the electron-transfer resistance. Those results suggest that a sol-gel derived graphite composite electrode might be utilized as a label-free electrochemical immunosensor for diagnosis, biochemical research, food industry, and so on.

Keywords:Immunosensor;Carbon Electrode;Sol-gel;E. coli O157:H7;Cyclic Voltammetry;Electrochemical Impedance Spectroscopy

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Liju Y, Yanbin L, Gisela FE, Anal. Chem., 76, 1107 (2004)
Joesph W, Prasad VAP, Kim RR, Anal. Chem., 70, 1171 (1998)
Tuzhi P, Quan C, Raymond CS, Anal. Chem., 72, 1611 (2000)
Kim YD, Park CB, Douglas SC, Biotechnol. Bioeng., 73, 331 (2001)
Oh BK, Lee WH, Choi JW, Biotech. Bioprocess Eng., 8, 227 (2003)
Chuanmin R, Liju Y, Yanbin L, Anal. Chem., 74, 4814 (2002)
Boubour E, Lennox RB, Langmuir, 16(9), 4222 (2000)
Markovich I, Mandler D, J. Electroanal. Chem., 484(2), 194 (2000)
Patolsky F, Filanovsky B, Katz E, Willner I, J. Phys. Chem. B, 102(50), 10359 (1998)
Allen JB, Larry RF, Electrochemical methods: Fundamentals and applications, 2nd, and applications, 2nd (2001)

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[1] Anand S, Joseph I, Thomas R, Biosens. Bioelectron., 21, 998 (2006)

[2] Jin C, Jinhai T, Feng Y, Huangxian J, Biomaterials, 27, 2313 (2006)

[3] Marta S, Francisco C, Salvador A, Esteve MF, Anal. Chem., 69, 2080 (1997)

[4] Fang AP, Ng H, Su XD, Li SFY, Langmuir, 16(12), 5221 (2000)

[5] Fang A, Ng HT, Li SFY, Langmuir, 17(14), 4360 (2001)

[6] Heyes CD, Kobitski AY, Amirgoulova EV, Nienhaus GU, J. Phys. Chem. B, 108(35), 13387 (2004)

[7] Ng HT, Fang AP, Huang LQ, Li SFY, Langmuir, 18(16), 6324 (2002)

[8] Wilhelm T, Wittstock G, Langmuir, 18(24), 9485 (2002)

[9] Albert FC, Dannyand KYW, Margaret CS, Anal. Chem., 71, 4088 (1999)

[10] Michael SW, Anal. Chem., 77, 1496 (2005)

[11] Mariela LO, Peter BH, Anal. Chem., 77, 5258 (2005)

[12] Peter F, Emerg. Infect. Dis., 1, 47 (1995)

[13] Yongcheng L, Yanbin L, Anal. Chem., 73, 5180 (2001)

[14] Lee W, Park KS, Kim YW, Lee YH, Choi JW, Biosens. Bioelectron., 20, 2292 (2005)

[15] Chuanmin R, Kefeng Z, Oomman KV, Craig AG, Anal. Chem., 75, 6494 (2003)

[16] Liju Y, Yanbin L, Gisela FE, Anal. Chem., 76, 1107 (2004)

[17] Joesph W, Prasad VAP, Kim RR, Anal. Chem., 70, 1171 (1998)

[18] Tuzhi P, Quan C, Raymond CS, Anal. Chem., 72, 1611 (2000)

[19] Kim YD, Park CB, Douglas SC, Biotechnol. Bioeng., 73, 331 (2001)

[20] Oh BK, Lee WH, Choi JW, Biotech. Bioprocess Eng., 8, 227 (2003)

[21] Chuanmin R, Liju Y, Yanbin L, Anal. Chem., 74, 4814 (2002)

[22] Boubour E, Lennox RB, Langmuir, 16(9), 4222 (2000)

[23] Markovich I, Mandler D, J. Electroanal. Chem., 484(2), 194 (2000)

[24] Patolsky F, Filanovsky B, Katz E, Willner I, J. Phys. Chem. B, 102(50), 10359 (1998)

[25] Allen JB, Larry RF, Electrochemical methods: Fundamentals and applications, 2nd, and applications, 2nd (2001)