The recent direct fabrication of micropatterned arrays of active biomolecules has received overwhelming attention due to its potential applications in biosensors and medical diagnostics. Here, we report an indirect soft-lithography-mediated method of fabricating hemoglobin (Hb) patterns in the submicrometer length scales region on indium-tin-oxide (ITO). Scanning electron microscopy, quartz crystal microbalance, and cyclic voltammetry studies reveal the formation of a self-assembled monolayer of Hb on an ITO surface. The present approach provides an easy and reliable means for simultaneous and defectless passivation and submicrometer patterning of proteins, thus indicating its potential application in biological assay.