Aflatoxin B1 was known to have strongest poisonous character among Aflatoxin compounds. To search the production of Aflatoxin B1 according to the change of temperature and relative humidity, melon incubated with Aspergillus parasiticus KCCM 35078 was incubated at 20°C, 25°C, 30°C, 35°C and at relative humidity 65%, 70%, 75%, 80% and for incubation period 4, 5, 6, 7, 8 days. Production of Aflatoxin B1 shows that the highest production appeared at 30°C, its relatiye humidity 75% and 7days. The result that was quantitaively ananlyzed by using HPLC was 43.34ppm, and by using Fluoroscence detector was 47.1ppm. Production of Aflatoxin B1 shows that the lowest production appeared at 25°C, its relative humidity 65% and 4days. The result that was quantitatively analyzed by using HPLC was 0.72ppm, and by using fluoroscence detector was 0.69ppm. The structure of Aflatoxin B1 was identify with authentic sample by using instrumental analysis methods of GC/MS, 1H-NMR, FT-IR. respectively. And, obserbed a total aspect of the pig carotid artery which a blood vessel of a person and similar structure for evaluate a characteristic of a blood bessel tissue reproduced according to decomposition by a biodegradable molecule layer of hybrid vascular gragft scaffold to electron microscopes. Therefore were able to confirm a blood vessel form to have consisted to three layer of the intima, media, adventitia which was basis structure composing a blood vessel tissue. Hybrid scafflod manufactured was confirmed, it is composed to ePTFE layer and two biodegradable poly(lactide-co-glycolide) layer in the middle, and having generally a uniformed concemtric circle. In the inner face of a hybrid biodegradable scaffold layer the figure that pore and node by porosity structure of the poly(lactide-co-glycolide) layer which composed a hybrid vascular graft inner layer were formed was obsered. However, as a result of having observed a hybrid media tissue revived for 1~8 weeks by smooth muscle cell, porosity structure that currently existed was not observed because of the media tissue which is revived.