화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.13, No.1, 39-44, February, 2005
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Preparation for Protein Separation of an Ion-Exchange Polymeric Stationary Phase Presenting Amino Acid and Amine Units Through Surface Graft Polymerization
Seong-Ho Choi†, Kwang-Pill Lee1, and Chang-Ho Shin2
  • Department of Chemistry, Hannam University, 133 Ojeng, Daeduck, Daejeon 306-791, Korea
  • 1Department of Chemistry Graduate School, Kyungpook National University, 1370 Sankyuk, Buk, Daegu 702-701, Korea
  • 2KT&G Central Research Institute, 302 Sinseong, Yuseong, Daejeon 305-345, Korea
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Ion-exchange polymeric stationary phases presenting amino acid and amino groups were prepared by the surface grafting of glycidyl methacrylate onto a silica gel surface and subsequent amination. Three kinds of amino acids-L-arginine (Arg), D-lysine (Lys), and D-histine (His)-were used in this study. An ion-exchange polymeric stationary phase presenting ethylene diamine (EDA) was also prepared by surface graft polymerization. Separation of the model proteins bovine serum albumin (BSA), chick egg albumin (CEA), and hemoglobin (Hb) was performed using the amino acid- and amine-derived columns. In separating the CEA/BSA mixture, the resolution time of BSA was longer than that of CEA when using the EDA column, whereas the resolution time of BSA was shorter than that of CEA when using the Arg, Lys, and His columns. In the separation of the Hb/BSA mixture, the resolution time of BSA was longer than that of Hb in the EDA column, whereas the resolution time of BSA was shorter than that of Hb in the amino acid columns (D-Lys, L-Arg, and D-His).
Keywords:ion-exchange polymeric stationary phase;glycidyl methacrylate (GMA);amino acid column;ethylene diamine column;hemoglobin (Hb);bovin serum albumin (BSA);chick egg albumin (CEA);resolution time
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