화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.109, 79-99, May, 2022
DOI10.1016/j.jiec.2022.02.028  Export Citation
Microfluidic free-flow electrophoresis: A promising tool for protein purification and analysis in proteomics
Youngbok Lee†, and Jae-Sung Kwon†
  • Department of Applied Chemistry, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 15588, Republic of Korea
E-mail:,
Microfluidic free-flow electrophoresis (μFFE) is the most promising technique for proteomics. This method can perform real-time separation and detection of analytes in a small device where a continuousflowof carrier buffer is driven and an external electric field is applied perpendicular to the buffer flow. The capability of μFFE has motivated extensive applications pertaining to the pre-fractionation, enrichment, and higherlevel purification of target proteins in biological systems. This review introduces the proteomics applications of the technique, along with a detailed theoretical overview, as follows. First, the principle and the band broadening involved in μFFE are explained. Next, materials for the fabrication of a μFFE device are described, followed by a summary of the online detection methods for μFFE. Finally, various applications of μFFE in proteomics fields are introduced, particularly focusing on microfluidic free-flow zone electrophoresis and microfluidic free-flow isoelectric focusing, the two major separation modes of μFFE.
Keywords:Microfluidic free-flow electrophoresis;Microfluidic free-flow zone electrophoresis;Microfluidic free-flow isoelectric focusing;Proteomics;Lab-on-a-chip system
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