화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.103, 381-391, November, 2021
DOI10.1016/j.jiec.2021.08.006  Export Citation
Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan
Esra Ilhan-Ayisigi1, 2, Gozde Budak3, 4, Melih Soner Celiktas5, Canan Sevimli-Gur3, and Ozlem Yesil-Celiktas1, †
  • 1Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova-Izmir, Turkey
  • 2Genetic and Bioengineering Department, Faculty of Engineering and Architecture, Ahi Evran University, 40200 Kirsehir, Turkey
  • 3Department of Biology, Biotechnology Discipline, Science and Arts Faculty, Kocaeli University, 41380 Izmit, Kocaeli, Turkey
  • 4YUTEG Biotechnology Pharmaceuticals and Food Supplements Limited Company, 34940 Tuzla Istanbul, Turkey
  • 5Solar Energy Institute, Ege University, 35100 Bornova-Izmir, Turkey
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Cereal grain-derived protein hydrolysates exert beneficial effects for human health, thus utilization of byproducts with high protein content has drawn attention. In this study, hot water extraction of rice husk has been optimized to obtain protein hydrolysate with the highest anticancer activity. The optimum protein value was obtained as 2.40 g/L corresponding to 43.2 g protein/kg dried rice husk under the conditions of 60 °C, 2.0 mL/min flow rate and pH 10.0. The protein hydrolysate was encapsulated with chitosan, where the mean particle size of protein hydrolysate (0.3 %) loaded chitosan nanoparticles was 256.4 ± 33. 4 nm with 89 % encapsulation efficiency and a 65% release at the end of 6 days. Cytotoxicity assays showed that the lowest cell viabilities have been achieved with A549 and MCF7 cells with IC50 values of 1.98 and 3.58 μg/mL, respectively, whereas nuclear fragmentation and apoptotic bodies were observed through Hoechst 33342 staining. The cytotoxic effect might be associated with the wide variety of peptide/ protein subunits ranging from 10 kDa to more than 180 kDa in the protein hydrolysate of rice husk.
Keywords:Peptide;Rice husk;Hot water extraction;Cytotoxicity;Chitosan nanoparticle
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