박테리아 셀룰로오스를 이용한 카본 퀀텀닷 합성과 응용

이동호; Dong Ho Lee

Polymer(Korea), Vol.43, No.5, 722-727, September, 2019

DOI10.7317/pk.2019.43.5.722 Export Citation

박테리아 셀룰로오스를 이용한 카본 퀀텀닷 합성과 응용

Synthesis and Application of Carbon Quantum Dot Using Bacterial Celluloses

이동호^†

중앙대학교 자연과학대학 생명과학과

Dong Ho Lee^†

Department of Life Science, College of Natural Science, Chung-Ang University, Seoul 06974, Korea

E-mail:

초록

본 연구에서는 높은 기계적 강도와 결정화도를 가지며, 생체 적합성이 뛰어난 박테리아 셀룰로오스(bacteria cellulose, BC)를 제조하였으며 BC를 200 ℃에서 열 분해하여 카본 퀀텀닷(carbon quantum dot, CQD)을 합성하였고, 형광 분광 광도계(fluorescence spectrophotometry)를 이용하여 BC-CQD의 형광 특성을 확인하였다. 합성된 BC-CQD 용액을 파장 365 nm에서 여기시켰을 때, 방출 파장 440 nm에서 최대 형광 방출이 나타남을 알 수 있었다. 이러한 측정 결과는 방출 파장 이 440-550 nm 안에 있는 기존 식물성 셀룰로오스를 이용한 CQD와 유사한 특성을 나타내었고, 여기 파장 변화에 따른 방출 파장 및 형광 강도의 변화를 측정한 결과 합성된 BC-CQD의 광학적 특성이 여기 파장 의존성을 나타냄을 관찰할 수 있었다. 또한 poly(vinyl alcohol)(PVA)와 복합화하여 제작한 BC-CQD/PVA 필름은 7일 이후에도 우수한 형광 안정성이 유지됨을 확인하였고 시차 열 중량분석(thermogravimetric analysis, TGA) 측정을 통하여 BC-CQD/PVA 필름의 열 안정성을 확인함으로써, 활용성이 다양한 멤브레인이나 전기화학발광(electrochemi-luminescence, ECL) 진단 및 바이오 분야에 이용될 수 있는 가능성을 제시하였다.

In this study, bacterial cellulose (BC) with high mechanical strength, crystallinity, and excellent biocompatibility was prepared. BC-carbon quantum dots (BC-CQD) were synthesized by thermal pyrolysis at 200 ℃. Fluorescence properties of BC-CQD solution were assessed by fluorescence spectrophotometry. BC-CQD solution was excited at 365 nm, and emission-maxima of the solution was at 440 nm. The optical characteristics of synthesized BC-CQD showed excitation wavelength-dependent emission wavelength-pattern as the emission intensity varied with excitation wavelength. These results showed similar excitation wavelength range, 440-550 nm, as observed for CQD with conventional plant celluloses. In addition, BC-CQD/poly(vinyl alcohol) (PVA) composite film exhibited extended fluorescence stability, for more than 7 days. The thermal stability of BC-CQD was demonstrated to be lower than that of PVA film through the weight loss estimation of BC-CQD/PVA film by thermogravimetric analysis. Therefore, it can potentially be applied as membrane material, in electrochemiluminescence (ECL) diagnosis and in biomedical fields.

Keywords:bacterial cellulose;carbon quantum dot;BC-CQD/PVA composite film;fluorescence stability

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[2] Molaei MJ, RSC Adv., 9, 6460 (2019)

[3] Xu XY, Ray R, Gu YL, Ploehn HJ, Gearheart L, Raker K, Scrivens WA, J. Am. Chem. Soc., 126(40), 12736 (2004)

[4] Wang X, Cao L, Lu F, Meziani MJ, Li H, Qi G, Zhou B, Harruff BA, Kermarrec F, Sun YP, Chem. Commun., 25, 3774 (2009)

[5] Zheng LY, Chi YW, Dong YQ, Lin JP, Wang BB, J. Am. Chem. Soc., 131(13), 4564 (2009)

[6] Yang ZC, Wang M, Yong AM, Wong SY, Zhang XH, Tan H, Chang AY, Li X, Wang J, Chem. Commun., 47, 11615 (2011)

[7] Hou J, Li H, Wang L, Zhang P, Zhou T, Ding H, Ding L, Talanta, 146, 34 (2016)

[8] Li H, He X, Liu Y, Huang H, Carbon, 49, 605 (2011)

[9] Feng Y, Zhong D, Miao H, Yang X, Talanta, 140, 128 (2015)

[10] Mehta VN, Jha S, Basu H, Singhal RK, Kailasa SK, Sens. Actuators B-Chem., 213, 434 (2016)

[11] Ng YH, Chin SF, Pang SC, Ng SM, Opt. Mater., 70, 50 (2017)

[12] Guo J, Liu D, Filpponen I, Johansson LS, Malho JM, Biomacromolecules, 18, 2014 (2017)

[13] Sun RC, BioRes., 4, 452 (2008)

[14] Park JK, Park YH, Jung JY, Biotechnol. Bioproc. E

[15] Czaja W, Romanovicz D, Brown RM, Cellulose, 11, 403 (2004)

[16] Esa F, Tasirin SM, Rahman NA, Agric. Agric. Sci. Procedia., 2, 113 (2014)

[17] Oksman K, Sain M, Editors, Cellulose nanocomposites: Processing, Characterization, and Properties (ACS symposium series 938), American Chemical Society, Washington DC, 2006.

[18] Tanpichai S, Quero F, Nogi M, Yano H, Young RJ, Lindstrom T, Sampson WW, Eichhorn SJ, Biomacromolecules, 13, 51340 (2012)

[19] Wu BN, Hu CY, Hu XQ, Cao HM, Huang CS, Shen HB, Jia NQ, Biosens. Bioelectron., 50, 300 (2013)

[20] Jie GF, Zhang J, Jie GX, Wang L, Biosens. Bioelectron., 52, 69 (2014)

[21] Liu ZY, Qi WJ, Xu GB, Chem. Soc. Rev., 44, 3117 (2015)

[22] Liu Z, Zhang X, Cui L, Wang K, Zhan H, Sens. Actuators B-Chem., 248, 402 (2017)

[23] Yoon OJ, Polym. Korea, 40(6), 998 (2016)

[24] Reiniati I, Dr. Thesis, The University of Western Ontario (2017).

[25] Li D, Ao K, Wang Q, Lv P, Wei Q, Molecules, 21, 618 (2016)

[26] Yoon OJ, Macromol. Res., 24(11), 973 (2016)

[27] Guo JQ, Liu DF, Filpponen I, Johanson LS, Malho JM, Quraishi S, Liebner F, Santos HA, Rojas OJ, Biomacromolecules, 18(7), 2045 (2017)

[28] Zhang YF, Maimaiti H, Zhang B, RSC Adv., 7, 2842 (2017)

[29] Hemalath KS, Parvatika N, Rukmani K, Int. J. Adv. Sci. Res., 5, 106 (2015)