pH-Selective Fluorescent Probe with Photothermal Ablation of Bacteria Based NIR Dye-Embedded Zwitterionic Carbon Dots

Eun Bi Kang; Pham Thi My Phuong; Gibaek Lee; Sangkug Lee; Insik In; Sung Young Park

Macromolecular Research, Vol.27, No.7, 720-728, July, 2019

DOI10.1007/s13233-019-7102-8 Export Citation

pH-Selective Fluorescent Probe with Photothermal Ablation of Bacteria Based NIR Dye-Embedded Zwitterionic Carbon Dots

Eun Bi Kang¹, Pham Thi My Phuong², Gibaek Lee¹, Sangkug Lee³, Insik In^{2, 4}, and Sung Young Park^{1, 2, †}

¹Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju, Chungbuk 27469, Korea
²Department of IT Convergence, Korea National University of Transportation, Chungju, Chungbuk 27469, Korea
³IT Convergence Material R&D Group, Korea Institute of Industrial Technology, Cheonan, Chungnam 31056, Korea
⁴Department of Polymer Science and Engineering, Korea National University of Transportation, Chungju, Chungbuk 27469, Korea

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We developed a highly pH-sensitive fluorescent carbon dot probe containing IR825 photothermal dye for rapid detection of environmental bacteria, with subsequent killing activity via near-infrared (NIR) driven photothermal ablation. The IR825-loaded fluorescent carbon dot (I-CD) interacted strongly with the negative charge of bacterial surface, expressed via fluorescence ON/OFF behavior under a broad range of pH conditions, by modifying the zwitterionic conformation of the carbon dot. In acidic and basic conditions, I-CD is activated, losing its hydrophobicity in neutral condition and releasing IR825, inducing NIR-mediated photothermal activity. Release of IR825 from the I-CD core contributed to high thermal conversion, killing bacterial cells with 99% efficiency. This material shows potential to be used as a convenient, rapid, low-cost, high-sensitivity fluorescent bacterial biosensor, combined with effective bacterial killing activity via photothermal conversion with pH and NIR triggers.

Keywords:bacteria detection;zwitterion;photothermal effect;fluorescent carbon dot

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[2] Petaccia M, Bombelli C, Sterbini FP, Papi M, Giansanti L, Bugli F, Sanguinetti M, Mancini G, Sens. Actuators B-Chem., 248, 247 (2017)

[3] Bhardwaj N, Bhardwaj SK, Nayak MK, Mehta J, Kim KH, Deep A, TrAC Trends Anal. Chem., 97, 120 (2017)

[4] Lam SJ, Wong EHH, Boyer C, Qiao GG, Prog. Polym. Sci, 76, 40 (2018)

[5] Ray PC, Khan SA, Singh AK, Senapati D, Fan Z, Chem. Soc. Rev., 8, 3193 (2012)

[6] Jin X, Yuan J, Shen J, Colloids Surf. B: Biointerfaces, 145, 275 (2016)

[7] Padan E, Bibi E, Ito M, Krulwich TA, BBA-Biomembranes, 1717, 67 (2005)

[8] Jose LM, Kuriakose S, Macromol. Res., 27, 73 (2018)

[9] Mazrad ZAI, In I, Lee KD, Park SY, Biosens. Bioelectron., 89, 1026 (2017)

[10] Kim YK, Kang EB, Kim SH, Sharker SM, Kong BY, In I, Lee KD, Park SY, ACS Appl. Mater. Interfaces, 8, 29827 (2016)

[11] Choi Y, Thongsai N, Chae A, Jo S, Kang EB, Paoprasert P, Park SY, In I, J. Ind. Eng. Chem., 47, 329 (2017)

[12] Kang EB, Mazrad ZIA, Robby AI, In I, Park SY, Eur. Polym. J., 105, 217 (2018)

[13] Kim SH, Kang EB, Jeong CJ, Sharker SM, In I, Park SY, ACS Appl. Mater. Interfaces, 7, 15600 (2015)

[14] Kang EB, In IS, Lee KD, Park SY, J. Ind. Eng. Chem., 55, 224 (2017)

[15] Zou L, Wang H, He B, Zeng L, Tan T, Cao H, He X, Zhang Z, Guo S, Li Y, Theranostics, 6, 762 (2016)

[16] Bao Z, Liu X, Liu Y, Liu H, Zhao K, Asian J. Pharm. Sci., 11, 349 (2016)

[17] Liu X, Yang G, Zhang L, Liu Z, Cheng Z, Zhu X, Nanoscale, 8, 15323 (2016)

[18] Zhang Y, Ang CY, Zhao Y, Polym. J., 48, 589 (2016)

[19] Feng L, Tong C, He Y, Liu B, Wang C, Sha J, Lu C, J. Lumines., 146, 502 (2014)

[20] Choi CA, Lee JE, Mazrad ZAI, In I, Jeong JH, Park SY, J. Ind. Eng. Chem., 63, 208 (2018)

[21] Taya P, Maiti B, Kumar V, De P, Satapathi S, Sens. Actuators B-Chem., 255, 2628 (2018)

[22] Khoerunnisa, Mazrad ZAI, In I, Park SY, Biosens. Bioelectron., 90, 394 (2017)

[23] Cheng L, He WW, Gong H, Wang C, Chen Q, Cheng ZP, Liu Z, Adv. Funct. Mater., 23(47), 5893 (2013)

[24] Kim YK, Kang EB, Kim SM, Park CP, In I, Park SY, ACS Appl. Mater. Interfaces, 9, 3192 (2017)

[25] Song X, Gong H, Liu T, Cheng L, Wang C, Sun X, Liang C, Liu Z, Small, 10, 4362 (2014)

[26] Zhang J, Feng YH, Mi JL, Shen YT, Tu ZG, Liu L, J. Hazard. Mater., 342, 121 (2018)

[27] Yang C, Xie H, Li QC, Sun EJ, Su BL, J. Colloid Interface Sci., 450, 388 (2015)

[28] Yuan H, Liu Z, Liu L, Lv F, Wang Y, Wang S, Adv. Mater., 26, 4333 (2015)

[29] Dakal TC, Kumar A, Majumdar RS, Yadav V, Front. Microbiol., 7, 1831 (2016)

[30] Malanovic N, Lohner K, Pharmaceuticals, 9, 1 (2016)

[31] Altintas Z, Akgun M, Kokturk G, Uludag Y, Biosens. Bioelectron., 100, 541 (2018)

[32] Hayden SC, Zhao GX, Saha K, Phillips RL, Li XN, Miranda OR, Rotello VM, El-Sayed MA, Schmidt-Krey I, Bunz UHF, J. Am. Chem. Soc., 134(16), 6920 (2012)