The MOFs (ZIF-8) coated Fe3O4/PPy magnetic nanocomposite (Fe3O4/PPy@ZIF-8) with large surface area and high conductivity was successfully prepared by polymerizing the pyrrole on Fe3O4 nanoparticle and self-assembling the ZIF-8 on the composite. Due to the outstanding specific area and pore volume of MOFs on the resulted nanocomposite, the glucose oxidase (GOx) could be effectively immobilized on the Fe3O4/PPy@ZIF-8 to fabricate a novel amperometric glucose biosensor. The as-prepared GOx/Fe3O4/PPy@ZIF-8/GCE biosensor exhibited extraordinary electro-detection performance for glucose with wide linear range from 1 mu M to 2 mM and the limit of detection was 0.333 mu M (S/N = 3). Furthermore, the prepared biosensor also showed good selectivity for glucose detection and satisfactory result in real samples detection.