Novel redox-active materials with the high surface area have great potential for electrochemical biosensors. In this research, we demonstrate the utilization of Ni-2(dihydroxyterephthalic acid) (also known as CPO-27-Ni-II and MOF-74-Ni-II) metal-organic framework for non-enzymatic detection of glucose. CPO-27-Ni-II is synthesized by ultrasound assisted solvothermal method with high purity, crystallinity, and large surface area. The CPO-27-Ni-II-modified glassy carbon electrode (GCE) shows excellent redox activity in the alkaline medium due to the formation of Ni-II/Ni-III redox pair in CPO-27-Ni-II. This corroborates the direct oxidation of glucose to gluconolactone. The sensor exhibits wide linear range with high sensitivity (ca. 40.95 mu A/mM), and low method detection limit (MDL) (ca. 1.46 mu M). The justification of this sensing platform was evaluated by standard addition method. Thus, the present biosensor could be used for the point of care detection of glucose.