Selective recovery of cadaverine from lysine decarboxylase bioconversion solution using methyl ethyl ketone

Yun-Gi Hong; Hyun-Joong Kim; Jong-Min Jeon; Yu-Mi Moon; Ju-Won Hong; Jeong-Chan Joo; Bong-Keun Song; Kyung-Mooon Park; Sang-Hyun Lee; Yung-Hun Yang

Journal of Industrial and Engineering Chemistry, Vol.64, 167-172, August, 2018

Yun-Gi Hong¹, Hyun-Joong Kim¹, Jong-Min Jeon¹, Yu-Mi Moon¹, Ju-Won Hong¹, Jeong-Chan Joo², Bong-Keun Song², Kyung-Mooon Park³, Sang-Hyun Lee¹, and Yung-Hun Yang^{1, 4, †}

¹Department of Microbial Engineering, College of Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea
²Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, Taejon 305-600, Republic of Korea
³Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong City, Republic of Korea
⁴Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul 143-701, Republic of Korea

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Efficient extraction and purification process is still a major bottleneck for economical production of cadaverine. We examined 10 different organic solvents to determine which one might be a suitable solvent for cadaverine extraction. Methyl ethyl ketone (MEK) was a very selective solvent for cadaverine. High pH was critical factor for cadaverine extraction with high purity. Cadaverine extraction efficiency of more than 70.1% with 99% of purity was successfully achieved by applying optimized extraction condition (pH 13.5, 58 °C, 200 rpm incubation for 6 h). Cadaverine extraction using MEK seems quite feasible and promising for the preparation of polyamide monomers for environmental process.

Keywords:Liquid.liquid extraction;Methyl ethyl ketone;Lysine decarboxylase;Cadaverine;Escherichia coli

[References]

Sabo DL, Boeker EA, Byers B, Waron H, Fischer EH, Biochemistry, 13, 662 (1974)
Yamamoto Y, Miwa Y, Miyoshi K, Furuyama J, Ohmori H, Genes Genet. Syst., 72, 167 (1997)
Qian ZG, Xia XX, Lee SY, Biotechnol. Bioeng., 108(1), 93 (2011)
Buschke N, Becker J, Schafer R, Kiefer P, Biedendieck R, Wittmann C, Biotechnol. J., 8, 557 (2013)
Buschke N, Schroder H, Wittmann C, Biotechnol. J., 6, 306 (2011)
Kim HJ, Kim YH, Shin JH, Bhatia SK, Sathiyanarayanan G, Seo HM, Choi KY, Yang YH, Park K, J. Microbiol. Biotechnol., 25, 1108 (2015)
Ma WC, Cao WJ, Zhang H, Chen KQ, Li Y, Ouyang PK, Biotechnol. Lett., 37(4), 799 (2015)
Ma WC, Cao WJ, Zhang BW, Chen KQ, Liu QZ, Li Y, Ouyang PK, Sci. Rep. UK, 5 (2015)
Bhatia SK, Kim YH, Kim HJ, Seo HM, Kim JH, Song HS, Sathiyanar-ayanan G, Park SH, Park K, Yang YH, Bioprocess Biosyst. Eng., 38, 2315 (2015)
Xiucai L, Liu C, Duanfang D, Qin B, Naiqiang L, Purification of cadaverine, Google Patents (2017).
Hanson C, Recent Advances in Liquid.Liquid Extraction, Elsevier, 2013.
Kind S, Neubauer S, Becker J, Yamamoto M, Volkert M, Abendroth G, Zelder O, Wittmann C, Metab. Eng., 25, 113 (2014)
Malinowski JJ, Biotechnol. Tech., 13, 127 (1999)
Tamada JA, King C, Extraction of Carboxylic Acids by Amine Extractants, Lawrence Berkeley Lab, CA, USA, 1989.
Kind S, Wittmann C, Appl. Microbiol. Biotechnol., 91(5), 1287 (2011)
Kamlet MJ, Doherty RM, Abboud JL, Abraham MH, Taft RW, J. Pharm. Sci., 75, 338 (1986)
Taft RW, Abboud JLM, Kamlet MJ, Abraham MH, J. Solut. Chem., 14, 153 (1985)
Inci I, Biotechnol. Prog., 23(5), 1171 (2007)
Wasewar KL, Heesink AB, Versteeg GF, Pangarkar VG, J. Biotechnol., 97, 59 (2002)
Gani R, Jimenez-Gonzalez C, Kate AT, Crafts PA, Jones M, Powell L, Atherton JH, Cordiner JL, Chem. Eng. N. Y., 113, 30 (2006)
Meunier N, Drogui P, Montane C, Hausler R, Mercier G, Blais JF, J. Hazard. Mater., 137(1), 581 (2006)
Kertes A, King CJ, Biotechnol. Bioeng., 28, 269 (1986)
Kim JH, Seo HM, Sathiyanarayanan G, Bhatia SK, Song HS, Kim J, Jeon JM, Yoon JJ, Kim YG, Park K, Yang YH, J. Ind. Eng. Chem., 46, 44 (2017)

[Referenced By]

[1] Sabo DL, Boeker EA, Byers B, Waron H, Fischer EH, Biochemistry, 13, 662 (1974)

[2] Yamamoto Y, Miwa Y, Miyoshi K, Furuyama J, Ohmori H, Genes Genet. Syst., 72, 167 (1997)

[3] Qian ZG, Xia XX, Lee SY, Biotechnol. Bioeng., 108(1), 93 (2011)

[4] Buschke N, Becker J, Schafer R, Kiefer P, Biedendieck R, Wittmann C, Biotechnol. J., 8, 557 (2013)

[5] Buschke N, Schroder H, Wittmann C, Biotechnol. J., 6, 306 (2011)

[6] Kim HJ, Kim YH, Shin JH, Bhatia SK, Sathiyanarayanan G, Seo HM, Choi KY, Yang YH, Park K, J. Microbiol. Biotechnol., 25, 1108 (2015)

[7] Ma WC, Cao WJ, Zhang H, Chen KQ, Li Y, Ouyang PK, Biotechnol. Lett., 37(4), 799 (2015)

[8] Ma WC, Cao WJ, Zhang BW, Chen KQ, Liu QZ, Li Y, Ouyang PK, Sci. Rep. UK, 5 (2015)

[9] Bhatia SK, Kim YH, Kim HJ, Seo HM, Kim JH, Song HS, Sathiyanar-ayanan G, Park SH, Park K, Yang YH, Bioprocess Biosyst. Eng., 38, 2315 (2015)

[10] Xiucai L, Liu C, Duanfang D, Qin B, Naiqiang L, Purification of cadaverine, Google Patents (2017).

[11] Hanson C, Recent Advances in Liquid.Liquid Extraction, Elsevier, 2013.

[12] Kind S, Neubauer S, Becker J, Yamamoto M, Volkert M, Abendroth G, Zelder O, Wittmann C, Metab. Eng., 25, 113 (2014)

[13] Malinowski JJ, Biotechnol. Tech., 13, 127 (1999)

[14] Tamada JA, King C, Extraction of Carboxylic Acids by Amine Extractants, Lawrence Berkeley Lab, CA, USA, 1989.

[15] Kind S, Wittmann C, Appl. Microbiol. Biotechnol., 91(5), 1287 (2011)

[16] Kamlet MJ, Doherty RM, Abboud JL, Abraham MH, Taft RW, J. Pharm. Sci., 75, 338 (1986)

[17] Taft RW, Abboud JLM, Kamlet MJ, Abraham MH, J. Solut. Chem., 14, 153 (1985)

[18] Inci I, Biotechnol. Prog., 23(5), 1171 (2007)

[19] Wasewar KL, Heesink AB, Versteeg GF, Pangarkar VG, J. Biotechnol., 97, 59 (2002)

[20] Gani R, Jimenez-Gonzalez C, Kate AT, Crafts PA, Jones M, Powell L, Atherton JH, Cordiner JL, Chem. Eng. N. Y., 113, 30 (2006)

[21] Meunier N, Drogui P, Montane C, Hausler R, Mercier G, Blais JF, J. Hazard. Mater., 137(1), 581 (2006)

[22] Kertes A, King CJ, Biotechnol. Bioeng., 28, 269 (1986)

[23] Kim JH, Seo HM, Sathiyanarayanan G, Bhatia SK, Song HS, Kim J, Jeon JM, Yoon JJ, Kim YG, Park K, Yang YH, J. Ind. Eng. Chem., 46, 44 (2017)