We report the hydrophobic interaction of chitosan-zinc-tin oxide nanoparticle with bovine albumin serum (BSA). A new renewable material of chitosan (N-deacetylated derivative of chitin) was subjected to action with zinc oxide and tin oxide modifiers. The nanoparticles of chitosan-zinc-tin oxide have been successfully synthesized by simple chemical precipitation method. The synthesized nanoparticles were characterized and confirmed by FTIR, XRD, HR-SEM with EDAX, TEM and BET analysis. This result clearly indicates that the colloidal nanoparticles are quenches the fluorescence of BSA through the ground state complex formation via static quenching mechanism. The quenching rate constant, binding sites, binding force and binding constant (K) = 0.80-1.41 x 10(4) M-1 were calculated from fluorescence spectral data and shows that the hydrophobic interaction is observed between colloidal nanoparticle and BSA. Thus the efficient binding of BSA with colloidal chitosan-zinc-tin oxide nanoparticle leads to play an important role in biomedical, biosensors and pharmaceutical applications. (C) 2017 Elsevier B.V. All rights reserved.