연골 재생을 위한 젤라틴과 젤란검을 혼합한 하이드로젤 평가

송정은; 송야성; 전성현; 최일남; 김초민; 강길선; Jeong Eun Song; Yah Seong Song; Sung Hyun Jeon; Il Nam Choi; Cho Min Kim; Gilson Khang

Polymer(Korea), Vol.41, No.4, 619-623, July, 2017

DOI10.7317/pk.2017.41.4.619 Export Citation

연골 재생을 위한 젤라틴과 젤란검을 혼합한 하이드로젤 평가

Evaluation of Gelatin and Gellan Gum Blended Hydrogel for Cartilage Regeneration

송정은, 송야성, 전성현, 최일남, 김초민, 강길선 ^†

전북대학교 BIN융합공학과, 고분자나노공학과, 고분자융합소재연구소

Jeong Eun Song, Yah Seong Song, Sung Hyun Jeon, Il Nam Choi, Cho Min Kim, and Gilson Khang^†

Dept. of BIN Convergence Tech & Dept. of PolymerNanoSci Tech, Chonbuk National University, 567 Baekje-daero, Jeonju 54898, Korea

E-mail:

초록

천연 다당류인 젤란검은 내열성, 내산성, 내효소성이 우수하여, 널리 조직공학적으로 응용을 위해 사용된다. 그러나 젤란검은 낮은 세포 부착력을 갖는 단점이 있다. 따라서 본 연구에서는 연골 세포 부착 효율을 증진시키기 위해, 젤란검 무게당 0, 10, 20 및 30 wt%의 젤라틴이 포함된 젤라틴/젤란검(G/GG) 하이드로젤을 제작하였다. 제작된 G/GG 하이드로젤의 물리적, 화학적 특성을 평가하기 위해, 압축강도, SEM, FTIR을 평가하였다. 또한 하이드로젤에서 연골 세포의 세포 부착력 및 기능적 유지를 관찰하기 위하여, MTT, RT-PCR을 수행하였다. 그 결과, 21일에 20 wt% G/GG 하이드로젤의 연골 세포 증식률은 0, 10, 및 30 wt%의 G/GG 하이드로젤보다 1.058, 1.051 및 1.04배 높게 나타났으며, 타입 2 콜라겐 유전자 발현 역시 각각 1.35, 1.21 및 1.16배로 관찰되었다. 그러므로, 20 wt%G/GG 하이드로젤은 연골 재생을 위한 생체재료로 활용할 수 있을 것으로 기대된다.

Gellan gum is a natural polysaccharide with good heat, acid and enzymes resistance, and widely used for tissue engineering application. However, one of the drawbacks of gellan gum is lower cell attachment. Hence, in this study, gelatin/ gellan gum (G/GG) hydrogels containing 0, 10, 20, and 30 wt% of gelatin per gellan gum weight were prepared for improvement of chondrocyte attachment efficiency. The mechanical and chemical properties of prepared G/GG hydrogel were evaluated using compression strength, scanning electron microscopy and FTIR. Also, the cellular proliferation and functional maintenance of chondrocytes in hydrogel was assessed by MTT analysis, and RT-PCR. The results showed that 20 wt% G/GG hydrogel was 1.058, 1.051 and 1.04 folds higher chondrocyte proliferation compared to 0, 10 and 30 wt% and Type-II collagen gene expression was 1.35, 1.21 and 1.16 folds at 21 days, respectively. Thus, 20 wt% G/GG hydrogel can be envisioned as a useful biomaterial for future cartilage regeneration applications.

Keywords:gellan gum;gelatin;hydrogel;cartilage regeneration

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de Oliveira Cardoso VM, Cury BSF, Evangelista RC, Gremiao MPD, J. Mech. Behav. Biomed. Mater., 65, 317 (2017)
Wang F, Wen Y, Bai TC, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 69, 268 (2016)
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