The objective of this work was to evaluate the technical feasibility of using both oil-extracted microalgae (M) and glycerol (G) in co-digestion with chicken litter (CL), thereby improving biochemical methane potential (BMP). Different feedstock ratios of M (0-30%), G (0-3%) and CL (67-100%) were investigated to determine the best co-digestion condition under mesophilic conditions. According to the modified Gompertz model, the best BMP (131.1 mL CH4 g(VSfed)(-1)) was obtained with the triple co-digestion (M: G: CL) in a proportion of 30:3:67. This yielded a methane production rate (mu m) of 3.3 mL CH4 g(VSfed)(-1) d(-1) and a lag time (lambda) of 17.4 d. This treatment reduced chemical oxygen demand (COD) by 91.02% and increased the methane yield 15.8% with respect to the CL control. (C) 2016 Elsevier Ltd. All rights reserved.