In situ transesterification of oleaginous yeast wet biomass for fatty acid methyl esters (FAMEs) production using acid catalyst, methanol with or without N-Lauroyl sarcosine (N-LS) treatment was performed. The maximum FAMEs yield obtained with or without N-LS treatment in 24 h reaction time was 96.1 +/- 1.9 and 71 +/- 1.4% w/w, respectively. The N-LS treatment of biomass followed by with or without ultrasonication revealed maximum FAMEs yield of 94.3 +/- 1.9% and 82.9 +/- 1.8% w/w using methanol to lipid molar ratio 360: 1 and catalyst concentration 360 mM (64 mu L H2SO4/g lipid) within 5 and 25 min reaction time, respectively. The FAMEs composition obtained in in situ transesterification was similar to that obtained with conventional two step lipid extraction and transesterification process. Biodiesel fuel properties (density, kinematic viscosity, cetane number and total glycerol) were in accordance with international standard (ASTM D6751), which suggests the suitability of biodiesel as a fuel. (C) 2016 Elsevier Ltd. All rights reserved.