화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.41, No.1, 1-6, January, 2017
DOI10.7317/pk.2017.41.1.1  Export Citation
농도별 실크스펀지에 따른 피부재생 영향 평가
Effect of Silk Sponge Concentrations on Skin Regeneration
신재훈, 이대훈, 송정은, 조성준, 박찬흠1, 강길선†
  • 전북대학교 BIN융합공학과, 고분자나노공학과, 고분자융합소재연구소
  • 1한림대학교 의과대학 춘천성심병원 이비인후과
Jae Hun Shin, Dae Hoon Lee, Jeong Eun Song, Sung Jun Cho, Chan Hum Park1, and Gilson Khang†
  • Dept. of BIN Convergence Tech & Dept. of PolymerNanoSci Tech, Chonbuk National University, 567 Baekje-daero, Jeonju 54898, Korea
  • 1Dept. of Otorhinolarygology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, 77 Sakju-ro, Chuncheon 24253, Korea
E-mail:
초록
실크는 천연 생체재료로, 낮은 염증성, 우수한 생체적합성을 가지고 있으며, 다양한 형태로 제조할 수 있다. 본 연구에서는 피부 재생에 있어, 실크 지지체의 효과를 확인하고자 in vitro와 in vivo 환경에서 실험을 진행하였다. 실크 지지체는 1, 2 및 3%의 스펀지 형태로 동결건조하여 제조하였고, 실크 지지체의 특성을 위해 SEM, FTIR 분석을 실시하였다. 세포 부착 및 증식률은 MTT 분석, 염증반응은 RT-PCR을 통해 확인하였다. 또한 피부재생 효과는 전층 결손을 유발시킨 마우스 모델에 실크 지지체를 이식한 뒤 적출하여, 조직학적 평가를 통해 확인하였다. 그 결과, 상처 치료에 있어, 실크 지지체의 응용 가능성을 확인하였다.
Silk is a well-studied biomaterial, possessing low immunity, high biocompatibility, and easy fabrication into various forms for several clinical applications. Herein, we have performed an in vitro (NIH/3T3 cells) and in vivo (rat models) study on silk sponges’ efficacy for skin regulation. The silk sponges were fabricated with 1, 2 and 3% silk contents via freeze-drying. All the samples physical characteristics were examined by FESEM and FTIR. In vitro biological properties including cell adhesion, proliferation and inflammation were confirmed by MTT assay and RT-PCR. Furthermore, the skin regeneration efficiency was examined by histological assessment, where the silk sponges were implanted at full thickness skin wounds in rat models. Considering all the obtained results, the silk sponges can be envisioned as a potential biomaterial for wound healing applications.
Keywords:skin regeneration;silk;inflammatory responses;scaffold
  1. Friess W, Eur. J. Pharm. Biopharm., 45, 113 (1998)
  2. Ng KW, Inter. J. Tissue Regen., 4, 29 (2013)
  3. Hollister SJ, Nat. Mater., 4(7), 518 (2005)
  4. Leal-Egana A, Scheibel T, Biotechnol. Appl. Biochem., 55, 155 (2010)
  5. Metcalfe AD, Ferguson MW, J. R. Soc. Interface, 4, 413 (2007)
  6. Lee OJ, Lee JM, Jin HJ, Park CH, Inter. J. Tissue Regen., 1, 68 (2010)
  7. Wood FM, Intl. J. Biochem. Cell Biol., 56, 133 (2014)
  8. Xu S, Sang L, Zhang Y, Wang X, Li X, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 33, 648 (2013)
  9. Lee W, Debasitis JC, Lee VK, Lee JH, Fischer K, Edminster K, Park JK, Yoo SS, Biomaterials, 30, 1587 (2009)
  10. Han F, Dong Y, Su Z, Yin R, Song AH, Li SM, Int. J. Pharm., 476, 124 (2014)
  11. Gerstenhaber JA, Brodsky R, Huneke RB, Lelkes PI, Wound Medicine, 5, 9 (2014)
  12. Ye Q, Ohsaki K, Li K, Li DJ, Zhu CS, Ogawa T, Tenshin S, Takano-Yamanoto T, Auris Nasus Larynx, 28, 131 (2007)
  13. Liu C, Xia Z, Czernuszka JT, Chem. Eng. Res. Des., 85(A7), 1051 (2007)
  14. Singer AJ, Clark RA, N. Engl. J. Med., 341, 738 (1999)
  15. Caliari SR, Ramirez MA, Harely BAC, Biomaterials, 32, 8990 (2011)
  16. Aszodi A, Legate KR, Nakchbandi I, Fassler R, Annu. Rev. Biochem., 22, 591 (2006)
  17. Song JE, Kim R, Lee JH, Kim HM, Kim CM, Lee D, Khang G, Intl. J. Tissue Regen., 4, 17 (2013)
  18. Brinckmann J, Top. Curr. Chem., 247, 1 (2005)
  19. Boehler RM, Shin S, Fast AG, Gower RM, Shea LD, Biomaterials, 34, 5431 (2013)
  20. Powell HM, Supp DM, Boyce ST, Biomaterials, 29, 834 (2008)
  21. Qian Y, Li L, Jiang C, Xu W, Lv Y, Zhong L, Cai K, Yang L, Intl. J. Biol. Macromol., 79, 133 (2015)
  22. Song JE, Kim AR, Lee CJ, Tripathy N, Yoon KH, Lee D, Khang G, J. Biomater. Sci.-Polym. Ed., 26, 181 (2015)
  23. Moon BM, Kim DK, Park HJ, Ju HW, Lee OJ, Kim JH, Lee JM, Lee JS, Park CH, Macromol. Res., 22(12), 1268 (2014)
  24. Han KS, Ko HA, Jang NK, Song JE, Khang G, Macromol. Res., 22(12), 1253 (2014)
  25. Zhang YQ, Biotechnol. Adv., 20, 91 (2002)
  26. Mandal BB, Kundu SC, Biomaterials, 30, 2956 (2009)
  27. Gu BK, Kim MS, Park SJ, Kim CH, Intl. J. Tissue Regen., 2, 83 (2011)
  28. Sahoo S, Toh SL, Goh JC, Biomaterials, 31, 2990 (2010)
  29. Vasconcelos A, Freddi G, Cavaco-Paulo A, Biomacromolecules, 9(4), 1299 (2008)
  30. Rockwood DN, Preda RC, Yucel T, Wang X, Lovett ML, Kaplan DL, Nat. Protoc., 6, 1612 (2011)
  31. Dash BC, Mandal BB, Kundu SC, J. Biotechnol., 144, 321 (2009)
  32. Mobini S, Hoyer B, Solati-Hashjin M, Lode A, Nosoudi N, Samadikuchaksaraei A, Gelinsky M, J. Biomed. Mater. Res. A, 101, 2392 (2013)
  33. Jin HJ, Park J, Valluzzi R, Cebe P, Kaplan DL, Biomacromolecules, 5(3), 711 (2004)
  34. So JW, Jang JW, Kim SH, Choi JH, Rhee JM, Min BH, Khang G, Polym. Korea, 32, 6 (2008)