Polymer mixed matrix membranes are the state-of-the-art materials for membrane based pharmaceuticals and bio-macromolecule separation applications. The present work demonstrates the fabrication of PPSu (polyphenylsulfone) and SPPSu (Sulfonated polyphenylsulfone)/nanoparticle titanium oxide (TiO2) hollow fiber membranes by phase inversion technique. SPPSu/TiO2 hollow fiber membranes resulted on higher flux about 25.5% compare to neat membrane, and with improved thermal and mechanical properties. The tethered of nanoparticle TiO2 and sulfonation on polymer matrix tailors the surface chemistry of the composite hollow fiber membrane by altering the morphology and water permeability. Cross section morphology was analyzed using a scanning electron microscopy (SEM). Surface topography were scanned using atomic force microscope (AFM) and nanocomposite membrane resulted with smooth surfaces. The pH -dependent surface charge properties of the prepared hollow fibers were determined through electro kinetic analyzer, demonstrating that the sulfonated PPSu blend membrane surface was negatively charged. The prepared membranes were also subjected to rejection ability of varying molecular weights of proteins and assigned the molecular weight cut-off of the membranes. SPPSu/TiO2 mixed matrix membranes resulted in improved (Rt approximate to 22%) antifouling properties. (C) 2016 Elsevier B.V. All rights reserved.