Skin regeneration using duck’s feet derived collagen and poly(vinyl alcohol) scaffold

Jeong Eun Song; Nirmalya Tripathy; Jae Hun Shin; Dae Hoon Lee; Chan Hum Park; Gilson Khang

Macromolecular Research, Vol.24, No.4, 359-365, April, 2016

DOI10.1007/s13233-016-4051-3 Export Citation

Skin regeneration using duck’s feet derived collagen and poly(vinyl alcohol) scaffold

Jeong Eun Song , Nirmalya Tripathy, Jae Hun Shin, Dae Hoon Lee, Chan Hum Park¹, and Gilson Khang^†

Department of BIN Fusion Tech, Polymer Fusion Res Center & Dept of Polymer·Nano Sci Tech, Chonbuk National Univ, 567 Baekje-daero, Jeonju, Jeonbuk, 54898, Korea
¹Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, 77, Sakju-ro, Chuncheon, Gangwon, 24253, Korea

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In this study, we have designed scaffold by mixing collagen extracted from the duck’s flippers with poly(vinyl alcohol) (DC/PVA) via freeze-thawing and the as-prepared scaffold were evaluated for skin regeneration. The physical and chemical properties of the scaffolds were characterized using FTIR, SEM, compressive strength, degree of swelling, etc. The in vitro behavior (cell proliferation) was examined after cultured with fibroblasts through the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. The in vivo results demonstrated that the scaffolds implanted in rat induced full-thickness defect, confirmed by histological staining. Encompassing all results, the DC/PVA scaffolds can be anticipated as efficient platforms for skin regeneration facilitating required cell adhesion, growth and proliferation.

Keywords:duck’s feet derived collagen;poly(vinyl alcohol);scaffold;freezing thawing;skin regeneration

[References]

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