화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.24, No.2, 131-142, February, 2016
DOI10.1007/s13233-016-4017-5  Export Citation
Characterization and preparation of bio-tubular scaffolds for fabricating artificial vascular grafts by combining electrospinning and a co-culture system
Boram Lee1, 2, Muhammad Shafiq1, 3, Youngmee Jung1, 3, Jong-Chul Park2, and Soo Hyun Kim1, 4, †
  • 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Cheongryang, Seoul, 02792, Korea
  • 2Department of Medical Science, Yonsei University, Seoul, 03722, Korea
  • 3Korea University of Science and Technology, 176 Gajeong-dong, Yuseong-gu, Daejeon, 34113, Korea
  • 4KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Korea
E-mail:
Tissue-engineered vascular scaffolds provide a promising solution for the replacement of diseased vascular structures. However, a major challenge lies in enhancing endothelialization, host cell ingrowth, and angiogenesis. In this study, we investigated the feasibility of developing a bio-tubular scaffold from human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVEC) co-cultured on electrospun poly(L-lactide-co-ε-caprolactone) membranes to address these issues. Confluent layers of HDFs stimulated the organization of HUVECs into capillary-like networks in an indirect contact (two-dimensional) co-culture on membranes. Bio-tubular scaffolds fabricated from co-cultured membranes were either grown statically in vitro or implanted subcutaneously in severe combined immunodeficient mice for up to 4 weeks for biocompatibility evaluation and functional performance. In vitro examination of co-cultures on scaffolds showed collagen remodeling and an improvement in biomechanical properties up to day 14. Morphological analysis of in vitro grown bio-tubular scaffolds revealed good attachment and growth of both cell types. After one month, co-cultured scaffolds in vivo showed higher infiltration of host cells and collagen remodeling as compared to the HDF-seeded grafts. After 4 weeks, thin continuous layers of endothelial cells and smooth muscle cells were formed as shown by staining with an antibody specific for CD31and α-actin (α-SMA). On the contrary, HDF-seeded scaffolds remained free of α-SMA-positive cells at all time points, whereas few CD31+ cells appeared after 4 weeks. Thus, co-cultured membranes provide a solution for enhancing endothelialization, tissue regeneration, and growth in bio-tubular scaffolds and may have broader applications in regenerative medicine.
Keywords:angiogenesis;endothelial cells;electrospinning;vascular scaffolds;PLCL
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