The present study demonstrates the application of Al-Al electrode combination for electrochemical disinfection of biologically treated municipal wastewater (BTMW) of Sewage Treatment Plant. A glass chamber of 2 liter volume was used for the Electrochemical (EC) process using two electrode plates of aluminum (Al-Al) with DC power supply at different operating conditions of current density (CD), operating time (OT), inter electrode distance (IED), electrode area (EA), initial pH, and settling time (ST). The maximum removal of total coliforms (TC) (99.99%) and total bacteria (TB) (99.87%) from BTMW was achieved with the optimum operating conditions of CD (2.65 A/m(2)), OT (40 mins.), IED (0.5 cm), EA (160 cm(2)), initial pH (7.5), and ST (60 min.). The efficiency of the EC process for disinfection of BTMW was strongly affected by the CD, OT, IED, EA, pH, and ST. These effects can be explained by the formation of the reactive species such as OH-, and ClO(2)(-center dot)during the electrochemical process. Under optimal operating conditions, the operating cost was found to be 1.01 $/m(3) in terms of the electrode consumption (23.71 x 10(-5) kg Al/m(3)) and energy consumption (101.76 kwh/m(3)).