Silk fibroin film as an efficient carrier for corneal endothelial cells regeneration

Eun Young Kim; Nimalya Tripathy; Jin Young Park; Seon Eui Lee; Choun-Ki Joo; Gilson Khang

Macromolecular Research, Vol.23, No.2, 189-195, February, 2015

DOI10.1007/s13233-015-3027-z Export Citation

Silk fibroin film as an efficient carrier for corneal endothelial cells regeneration

Eun Young Kim, Nimalya Tripathy, Jin Young Park, Seon Eui Lee, Choun-Ki Joo¹, and Gilson Khang^†

Department of BIN Fusion Technology, Department of PolymerNano Science & Technology and Polymer BIN Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin, Jeonbuk, 561-756, Korea
¹Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Gangnam St. Mary Hospital, 505 Banpo-dong, Seocho-gu, Seoul, 137-701, Korea

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Tissue engineered corneas are a promising alternative to the limited donor corneas for transplantation. Here, we fabricated a transparent silk fibroin film with varied sericin content - low sericin silk fibroin film (LSS) and high sericin silk fibroin film (HSS) - to evaluate its ability to serve as an efficient carrier for corneal endothelial cells. Both LSS and HSS were prepared from Bombyx mori containing different percentages of sericin -6.25% and 9.83%, respectively. Tissue culture polystyrene was used as a reference substratum. In vitro studies, such as a morphological evaluation, initial attachment, expression of mRNAs and proteins (ZO-1 and Na+/K+-ATPase), were performed using rabbit primary corneal endothelial cells as models on silk fibroin films. Results showed that the corneal endothelial cells grown on as-fabricated LSS and HSS were well-maintained, indicating no effect of the difference in sericin content in the films. However, the HSS displayed better initial cell attachment due to the adhesive properties of sericin. These results demonstrated that rabbit corneal endothelial cells grew well on a silk substratum although the percentage of sericin showed no significant difference.

Keywords:corneal endothelial cells;fibroin;film;sericin;silk;tissue engineered substrata

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[4] Peh GSL, Toh KP, Wu FY, Tan DT, Mehta JS, PLoS ONE, 6, 28310 (2011)

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[6] Shima N, Kimoto M, Yamaguchi M, Yamagami S, Invest. Ophthalmol. Vis. Sci., 52, 8711 (2011)

[7] Nakahara M, Okumura N, Kay EDP, Hagiya M, Imagawa K, Hosoda Y, Kinoshita S, Koizumi N, PLoS ONE, 8, 69009 (2013)

[8] Lange TM, Wood TO, McLaughlin BJ, J. Cataract Refract. Surg., 19, 232 (1993)

[9] Joo CK, Green WR, Pepose JS, Fleming TP, Graefes Arch. Clin. Exp. Ophthalmol., 238, 174 (2000)

[10] Mimura T, Yamagami S, Yokoo S, Invest. Ophthalmol. Vis. Sci., 45, 2992 (2004)

[11] Ishino Y, Sano Y, Nakamura T, Connon CJ, Rigby H, Fullwood NJ, Kinoshita S, Invest. Ophthalmol. Vis. Sci., 45, 800 (2004)

[12] Choi JS, Williams JK, Greven M, Walter KA, Laber PW, Khang G, Soker S, Biomaterials, 31, 6738 (2010)

[13] Lee JH Kim EY, Lee CJ, Joo CK, Khang G, Int. J. Tissue Regen., 4, 53 (2013)

[14] Capelli R, Amsden JJ, Generali G, Toffanin S, Benfenati V, Muccini M, Kaplan DL, Omenetto FG, Zamboni R, Org. Electron., 12, 1146 (2011)

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[18] Lawrence BD, Marchant JK, Pindrus MA, Omenetto FG, Kaplan DL, Biomaterials, 30, 1299 (2009)

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[20] Chirila TV, Barnard Z, Zainuddin, Harkin D, Mater. Sci. Forum, 561-565, 1549 (2007)

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