화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.22, No.3, 297-303, March, 2014
DOI10.1007/s13233-014-2037-6  Export Citation
Fabrication of Transparent Silk Fibroin Film for the Regeneration of Corneal Endothelial Cells; Preliminary Study
Hyeon Yoon, Eun Young Kim, Hyeongseok Kim, Chan Hum Park1, Choun-Ki Joo2, and Gilson Khang†
  • Department of BIN Fusion Technology, Department of PolymerNano Science & Technology and Polymer BIN Research Center, Chonbuk National University, Jeonju, 561-756, Korea
  • 1Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Gangwon, 200-702, Korea
  • 2Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Gangnam St. Mary Hospital, Seoul, 137-701, Korea
E-mail:
Dystrophies related to the corneal endothelium result in edema, loss of transparency, and finally, blindness. Currently, the only treatment of patients with these corneal endothelial dystrophies is transplantation. However, the limited availability of donor corneas is a main obstacle to transplanting healthy endothelia. In this study, we have chosen silk from the silkworm to make a substratum for corneal endothelial cells (CEnCs). This approach offers the possibility of overcoming the issue of limited supply of donor corneas. Two types of transparent silk films with different sericin contents have been prepared by boiling for 30 min and 1 h, respectively, as delivery vehicles for application on the corneal endothelium. The contents of sericin for high-sericin silk (HSS) and low-sericin silk (LSS) are 9.83% and 6.25%, respectively. Physical and chemical properties were analyzed by contact angle, transparency, tensile strength, FTIR, and DSC. Proliferation assay by MTT was conducted to confirm the viability of the CEnCs. These results demonstrate that the fabricated transparent silk films have sufficient physical and chemical properties as a possible carrier for CEnCs.
Keywords:Bombyx mori;silk fibroin;transparent film;cornea endothelial cell.
  1. Joyce NC, Exp. Eye Res., 95, 16 (2012)
  2. Mimura T, Yokoo S, Yamagami S, J. Funct. Biomater., 3, 726 (2012)
  3. Aldave AJ, Sonmez B, Arch. Ophthalmol., 125, 177 (2007)
  4. Aldave AJ, Frausto JHRF, Clin. Genet. (2013)
  5. Sabater AL, Guarnieri A, Espana EM, Li W, Prosper F, Moreno-Montanes J, Regen. Med., 8, 183 (2013)
  6. Zavala J, Jaime GRL, Barrientos CAR, Valdez-Garcia J, Eye., 27, 579 (2013)
  7. Joyce NC, Harris DL, Markov V, Zhang Z, Saitta B, Mol. Vis., 18, 547 (2012)
  8. Wang JH, Hung CH, Young TH, Biomaterials., 27, 3441 (2006)
  9. Vepari C, Kaplan DL, Prog. Polym. Sci., 32, 991 (2007)
  10. Kim TH, Ko JH, Kim SJ, Park YH, Int. J. Tissue Regen., 2, 1 (2011)
  11. Moy RL, Lee A, Zalka A, Am. Fam. Physician., 44, 2123 (1991)
  12. Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M, Biosci. Biotechnol. Biochem., 62, 145 (1998)
  13. Shao Z, Vollrath F, Nature., 418, 741 (2002)
  14. Panilaitis B, Altman GH, Chen J, Jin H, Karageorgiou V, Kaplan DL, Biomaterials., 24, 3079 (2003)
  15. Arai T, Freddi G, Innocenti R, Tsukada M, J. Appl. Polym. Sci., 91(4), 2383 (2004)
  16. Meinel L, Hofmann S, Karageorgiou V, Kirker-Head C, McCool J, Gronowicz G, Zichner L, Langer R, Vunjak-Novakovic G, Kaplan DL, Biomaterials., 26, 147 (2005)
  17. Motta A, Fambri L, Migliaresi C, Macromol. Chem.Phys., 203, 1658 (2002)
  18. Minoura N, Tsukada M, Nagura M, Biomaterials., 11, 430 (1990)
  19. Lawrence BD, Cronin-Golomb M, Georgakoudi I, Kaplan DL, Omenetto FG, Biomacromolecules, 9(4), 1214 (2008)
  20. Li M, Tao W, Kuga S, Nishiyama Y, Polym. Adv. Technol., 14, 694 (2003)
  21. Lee OJ, Lee JM, Jin HJ, Park CH, Int. J. Tissue Regen., 1, 68 (2010)
  22. Yamamoto M, Tabata Y, Int. J. Tissue Regen., 4, 36 (2013)
  23. Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL, Biomaterials., 24, 401 (2003)
  24. Wang Y, Rudym DD, Walsh A, Abrahamsen L, Kim H, Kim HS, Kirker-Head C, Kaplan DL, Biomaterials., 29, 3415 (2008)
  25. Nishidaa A, Naganuma T, Kanazawa T, Takashima Y, Yamada M, Okada H, Int. J. Phytoremediation., 414, 193 (2011)
  26. Kaewkorn W, Limpeanchob N, Tiyaboonchai W, Pongcharoen S, Sutheerawa-ttananonda M, Biol. Res., 45, 45 (2012)
  27. Tsubouchi K, Ugarashi Y, Takasu Y, Yamada H, Biosci. Biotechnol. Biochem., 69, 403 (2005)
  28. Liu J, Lawrence BD, Liu A, Schwab IR, Oliveira LA, Rosenblatt MI, Invest. Ophthalmol. Vis. Sci., 53, 4130 (2012)
  29. Nazarov R, Jin HJ, Kaplan DL, Biomacromolecules, 5(3), 718 (2004)
  30. Mandal BB, Priya AS, Kundu SC, Acta Biomater., 5, 3007 (2009)
  31. Takeuchi A, Ohtsuki C, Miyazaki T, Kamitakahara M, Ogata S, Yamazaki M, Furutani Y, Kinoshita H, Tanihara M, J. R. Soc. Interace., 2, 373 (2005)
  32. Baba T, Hanada K, Hashimoto I, J. Dermatol. Sci., 12, 18 (1996)
  33. Zhaorigetu S, Yanaka N, Sasaki M, Watanabe H, Kato N, J. Photochem. Photobiol. B: Biol., 71, 11 (2003)
  34. Terada S, Nishimura T, Sasaki M, Yamada H, Miki M, Cytotechnology., 40, 3 (2002)
  35. Terada S, Sasaki M, Yanagihara K, Yamada H, J. Biosci. Bioeng., 100(6), 667 (2005)
  36. Freddi G, Mossotti R, Innocenti R, J. Biotechnol., 106, 101 (2003)
  37. Sah MK, Pramanik K, Int. J. Environ. Sci. Dev., 1, 404 (2010)
  38. Yang DH, Park HN, Lee JB, Heo DN, Bae MS, Kwon IK, Int. J. Tissue Regen., 2, 119 (2011)
  39. Khang G, Lee SJ, Jeon JH, Lee JH, Lee HB, Polym.(Korea), 24(6), 869 (2000)
  40. Zeng Y, Yang J, Huang K, Lee Z, Lee X, J. Biomech., 34, 533 (2001)
  41. Chu CC, Ann. Surg., 365 (1981)