다공성 고분자 마이크로겔의 Bovine Serum Albumin 단백질의 흡착평형

김공수; 강석호

Journal of the Korean Industrial and Engineering Chemistry, Vol.9, No.2, 311-316, April, 1998

Export Citation

다공성 고분자 마이크로겔의 Bovine Serum Albumin 단백질의 흡착평형

Adsorption Equilibrium of Bovine Serum Albumin Protein on Porous Polymer Microgels

김공수, 강석호

초록

물리 ·화학적인 특성이 다른 다공성 고분자 마이크로겔에 대한 bovine serum albumin(BSA-protein) 단백질의 흡착평형 특성을 연구하였다. 수용액 속에서 고분자와 단백질사이의 소수성 상호작용에 의하여 폴리부틸메타크릴레이트 (PBMA) 마이크로겔이 폴리비닐피리딘 (PVP)과 폴리아크릴로니트릴 (PAN) 마이크로겔보다 높은 흡착특성을 나타내었으며 PBMA 마이크로겔이 PVP와 PAN 마이크로겔보다 비가역적으로 흡착평형 특성을 나타내었다. 그러므로 고분자 마이크로겔의 물리적인 특성과 단백질-고분자 마이크로겔 사이의 정전기적 인력보다는 소수성 상호작용이 단백질의 흡착특성에 중요한 역할을 하고 있음을 알 수 있다. 또한 PBMA, PVP 및 PAN 마이크로겔 모두 Freundlich 흡착 등온식보다는 Langmuir 흡착 등온식에 잘 적용되었다.

The adsorption equilibrium properties of bovine serum albumin(BSA-protein) for three kinds of porous microgels with different physical and chemical features were investigated. The adsorption amount of BSA-protein on poly(butyl methacrylate)(PBMA) microgels was higher than those on poly(vinyl pyridine)(PVP) and poly(acrylonitrile)(PAN) microgels due to the hydrophobic interaction between polymer and protein in an aqueous solution. And PBMA microgels had more irreversible adsorption equilibrium properties the PVP and PAN microgels. It implies that hydrophobic interaction plays a more important role in adsorption properties of BAS-protein than physical properties of polymer and electrostatic attraction between protein and polymer microgels. Characteristics of the microgels used in this study followed Langmuir equation better than the Freundlich equation.

[References]

Ogino K, Sato H, 일본고분자논문집, 46, 667 (1989)
Kulin LI, Ellingsen T, Ugelstad J, J. Chromatogr., 514, 1 (1990)
Ellingsen T, Aune O, Ugelstad J, Hagen S, J. Chromatogr., 535, 147 (1990)
Cheng CM, Vanderhoff JW, El-Asser MS, J. Polym. Sci., 30, 245 (1992)
Gehrke SH, Andrews GP, Cussler EL, Chem. Eng. Sci., 41, 2153 (1986)
Kawaguchi H, Hoshinon F, Ohtsuka Y, Makromol. Chem. Rapid Commun., 7, 109 (1986)
Freitas RFS, Cussler EL, Chem. Eng. Sci., 42, 97 (1987)
Okubo M, Polym. Int., 30, 469 (1993)
Park TG, Hoffman AS, J. Biomed. Mater. Res., 24, 21 (1990)
Park TG, Hoffman AS, Biotechnol. Bioeng., 35, 152 (1990)
Egawa H, Nakayama M, Nonaka T, Uemura K, J. Appl. Polym. Sci., 34, 1557 (1987)
Kim KS, Kang SH, Park MH, Lee SH, J. Korean Ind. Eng. Chem., 5(6), 1009 (1994)
Kondo A, Oku S, Higashitani K, Biotechnol. Bioeng., 37, 537 (1991)
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Kitano H, Nakamura K, Biotechnol. Bioeng., 37, 608 (1991)
Yoshida H, Nishihara H, Biotechnol. Bioeng., 41, 280 (1993)
Yoshida H, Nishihara H, Kataoka T, Biotechnol. Bioeng., 43(11), 1087 (1994)
Kim KS, Kang SH, Shin JS, J. Ind. Eng. Chem., 2(1), 45 (1996)
Kim KS, Kang SH, Kim YS, J. Korean Ind. Eng. Chem., 7(4), 777 (1996)
Dubinin MM, Astakov VA, Adv. Chem., 102, 69 (1971)
Myers D, "Surfaces, Interfaces, and Colloids: Principles and Applications," Chap. 7, pp. 111-138, VCH Publishers, Inc., New York (1991)

[Referenced By]

[1] Ogino K, Sato H, 일본고분자논문집, 46, 667 (1989)

[2] Kulin LI, Ellingsen T, Ugelstad J, J. Chromatogr., 514, 1 (1990)

[3] Ellingsen T, Aune O, Ugelstad J, Hagen S, J. Chromatogr., 535, 147 (1990)

[4] Cheng CM, Vanderhoff JW, El-Asser MS, J. Polym. Sci., 30, 245 (1992)

[5] Gehrke SH, Andrews GP, Cussler EL, Chem. Eng. Sci., 41, 2153 (1986)

[6] Kawaguchi H, Hoshinon F, Ohtsuka Y, Makromol. Chem. Rapid Commun., 7, 109 (1986)

[7] Freitas RFS, Cussler EL, Chem. Eng. Sci., 42, 97 (1987)

[8] Okubo M, Polym. Int., 30, 469 (1993)

[9] Park TG, Hoffman AS, J. Biomed. Mater. Res., 24, 21 (1990)

[10] Park TG, Hoffman AS, Biotechnol. Bioeng., 35, 152 (1990)

[11] Egawa H, Nakayama M, Nonaka T, Uemura K, J. Appl. Polym. Sci., 34, 1557 (1987)

[12] Kim KS, Kang SH, Park MH, Lee SH, J. Korean Ind. Eng. Chem., 5(6), 1009 (1994)

[13] Kondo A, Oku S, Higashitani K, Biotechnol. Bioeng., 37, 537 (1991)

[14] Kitano H, Nakamura K, Biotechnol. Bioeng., 31, 547 (1988)

[15] Kitano H, Nakamura K, Biotechnol. Bioeng., 37, 608 (1991)

[16] Yoshida H, Nishihara H, Biotechnol. Bioeng., 41, 280 (1993)

[17] Yoshida H, Nishihara H, Kataoka T, Biotechnol. Bioeng., 43(11), 1087 (1994)

[18] Kim KS, Kang SH, Shin JS, J. Ind. Eng. Chem., 2(1), 45 (1996)

[19] Kim KS, Kang SH, Kim YS, J. Korean Ind. Eng. Chem., 7(4), 777 (1996)

[20] Dubinin MM, Astakov VA, Adv. Chem., 102, 69 (1971)

[21] Myers D, "Surfaces, Interfaces, and Colloids: Principles and Applications," Chap. 7, pp. 111-138, VCH Publishers, Inc., New York (1991)