실크필름에 배양한 망막색소상피세포의 거동

이소진; 김혜윤; 김슬지; 양재원; 이선의; 박찬흠; 주천기; 강길선; So Jin Lee; Hye Yun Kim; Seul Ji Kim; Jaewon Yang; Seon Ui Lee; Chan Hum Park; Choun-Ki Joo; Gilson Khang

Polymer(Korea), Vol.38, No.3, 364-370, May, 2014

Export Citation

실크필름에 배양한 망막색소상피세포의 거동

Behavior of Retinal Pigment Epithelial Cells Cultured on Silk Films

이소진, 김혜윤, 김슬지, 양재원, 이선의, 박찬흠 ¹, 주천기 ², 강길선 ^†

전북대학교 BIN 융합공학과, 고분자나노공학과
¹한림대학교 의과대학 춘천성심병원 이비인후과
²가톨릭 의과대학 안과학

So Jin Lee, Hye Yun Kim, Seul Ji Kim, Jaewon Yang, Seon Ui Lee, Chan Hum Park¹, Choun-Ki Joo², and Gilson Khang^†

Department of BIN Fusion Technology, Department of Polymer-Nano Science & Technology and Polymer BIN Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin, Jeonju 561-756, Korea
¹Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, 1-1 Okcheon, Chuncheon, Gangwon 200-702, Korea
²Department of Ophthalmology and Visual Science, The Catholic University of Korea, Seoul St. Mary's Hospital, College of Medicine, 505 Banpo-dong, Seocho-ku, Seoul 137-701, Korea

E-mail:

초록

망막색소상피(RPE)는 건강한 망막을 유지하는데 중요한 역할을 하고 RPE의 퇴화는 많은 망막질병을 유발한다. RPE 이식은 최근 망막 퇴화에 대한 가능성 있는 치료법으로 제시되고 있다. RPE 세포를 안전하게 이식하기 위해서는 지지체가 필요하므로 독특한 기계적 성질과 생체적합성을 갖는 실크를 사용하여 필름을 제조하였다. 실크필름의 FTIR, 접촉각 및 생분해성을 측정한 후, RPE 세포를 실크필름에 파종하여 그 영향을 확인하였다. MTT 분석, SEM, 면역형광염색, RT-PCR을 통해 세포의 부착, 생존도, 형태유지, 특이적 mRNA의 발현을 분석하였다. 본 연구에서는, 실크필름에 배양한 RPE 세포의 부착, 증식 및 표현형 유지가 뛰어남을 확인함으로써 실크필름의 망막 재생을 위한 조직 공학적 지지체로의 응용 가능성을 제시했다.

The retinal pigment epithelium (RPE) plays an important role in maintaining a healthy retina and the degeneration of RPE caused a number of retinal diseases. The transplantation of RPE has recently become a possible therapeutic modality for retinal degeneration. To transplant RPE cells securely, substrates are essential, and then as a substrate, we fabricated films using silk that has unique mechanical properties and biocompatibility. After the FTIR spectra, contact angle and biodegradation of silk films were confirmed, RPE cells were seeded and the influence of RPE cells on silk films was examined. We measured the cell adhesion, cell viability, morphology and specific mRNA expression by MTT assay, SEM, immunofluorescence and RT-PCR. In this study, we confirmed that attachment, proliferation and phenotype maintenance of RPE cells cultured on silk films were great, and thereby we were able to confirm the potential applications of silk films as tissue engineering carrier for regeneration of retina.

Keywords:retinal pigment epithelial cells;silk;film.

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Thumann G, Viethen A, Gaebler A, Walter P, Kaempf S, Johnen S, Salz AK, Biomaterials, 30, 287 (2009)
Horan RL, Antle K, Collette AL, Wang Y, Huang J, Moreau JE, Volloch V, Kaplan DL, Altman GH, Biomaterials, 26, 3385 (2005)
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[2] Lu JT, Lee CJ, Bent SF, Fishman HA, Sabelman EE, Biomaterials, 28, 1486 (2007)

[3] Shadforth AM, George KA, Kwan AS, Chirila TV, Harkin DG, Biomaterials, 33, 4110 (2012)

[4] Strauss O, Physiol. Rev., 85, 845 (2005)

[5] Kinnunen K, Petrovski G, Moe MC, Berta A, Kaarniranta K, Acta Ophthalmol., 90, 299 (2012)

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[8] Barone I, Novelli E, Piano I, Gargini C, Strettoi E, PLoS One, 7, 50726 (2012)

[9] Busskamp V, Duebel J, Balya D, Fradot M, Viney TJ, Siegert S, Groner AC, Cabuy E, Forster V, Seeliger M, Biel M, Humphries P, Paques M, Mohand-Said S, Trono D, Deisseroth K, Sahel JA, Picaud S, Roska B, Science, 329(5990), 413 (2010)

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[20] Wang Y, Rudym DD, Walsh A, Abrahamsen L, Kim HJ, Kim HS, Kirker-Head C, Kaplan DL, Biomaterials, 29, 3415 (2008)

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[28] Dash BC, Mandal BB, Kundu SC, J. Biotechnol., 144, 321 (2009)

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